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Product Info Summary
| SKU: | RP1073 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Toll-like receptor 8 TLR8 Antibody Picoband®
SKU/Catalog Number
RP1073
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Toll-like receptor 8 TLR8 Antibody catalog # RP1073. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Toll-like receptor 8 TLR8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1073)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TLR8, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1073 is reactive to TLR8 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
119828 MW
Background of TLR8
TLR8 (Toll-like receptor 8) is a protein that in humans is encoded by the TLR8 gene. TLR8 has also been designated as CD288 (cluster of differentiation 288).The TLR8 gene is mapped to Xp22.3-p22.2 by Chuang and Ulevitch (2000) and Du et al. (2000). The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. TLR8 recognises G-rich oligonucleotides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1073 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Liver Tissue, HEPG2 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-TLR8 antibody, RP1073, Western blotting
All lanes: Anti TLR8 (RP1073) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 120KD
Observed bind size: 120KD
Protein Target Info & Infographic
Gene/Protein Information For TLR8 (Source: Uniprot.org, NCBI)
Gene Name
TLR8
Full Name
Toll-like receptor 8
Weight
119828 MW
Superfamily
Toll-like receptor family
Alternative Names
Toll-like receptor 8;CD288;TLR8;UNQ249/PRO286; TLR8 CD288 toll like receptor 8 toll-like receptor 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR8, check out the TLR8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Toll-like receptor 8 TLR8 Antibody Picoband® (RP1073)
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No publications found for RP1073
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Customer Q&As
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16 Customer Q&As for Anti-Toll-like receptor 8 TLR8 Antibody Picoband®
Question
Is this RP1073 anti-TLR8 antibody reactive to the isotypes of TLR8?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The immunogen of RP1073 anti-TLR8 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TLR8 (881-907aa DAYISYDTKDASVTDWVINELRYHLEE), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-09
Question
I see that the anti-TLR8 antibody RP1073 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-08
Question
Is there a BSA free version of anti-TLR8 antibody RP1073 available?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TLR8 antibody RP1073 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-23
Question
Would anti-TLR8 antibody RP1073 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
According to the expression profile of placenta, TLR8 is highly expressed in placenta. So, it is likely that anti-TLR8 antibody RP1073 will work for WB with placenta.
Boster Scientific Support
Answered: 2020-03-17
Question
Does RP1073 anti-TLR8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
You can see on the product datasheet, RP1073 anti-TLR8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-24
Question
Please see the WB image, lot number and protocol we used for placenta using anti-TLR8 antibody RP1073. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-20
Question
We are currently using anti-TLR8 antibody RP1073 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The anti-TLR8 antibody (RP1073) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-09
Question
I am interested in using your anti-TLR8 antibody for cellular response to mechanical stimulus studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
I appreciate your inquiry. This RP1073 anti-TLR8 antibody is tested on rat liver tissue, tissue lysate, hepg2 whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-25
Question
Would anti-TLR8 antibody RP1073 work on pig WB with blood?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Our lab technicians have not tested anti-TLR8 antibody RP1073 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TLR8 antibody RP1073 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
I was wanting to use your anti-TLR8 antibody for WB for rat placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat placenta identification?
T. Evans
Verified customer
Asked: 2018-01-08
Answer
As indicated on the product datasheet, RP1073 anti-TLR8 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-08
Question
We have seen staining in human liver. Are there any suggestions? Is anti-TLR8 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
From what I have seen in literature liver does express TLR8. From what I have seen in Uniprot.org, TLR8 is expressed in blood, placenta, liver, among other tissues. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425
Boster Scientific Support
Answered: 2017-12-18
Question
Is a blocking peptide available for product anti-TLR8 antibody (RP1073)?
Verified Customer
Verified customer
Asked: 2017-08-23
Answer
We do provide the blocking peptide for product anti-TLR8 antibody (RP1073). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-23
Question
We need to test anti-TLR8 antibody RP1073 on rat placenta for research purposes, then I may be interested in using anti-TLR8 antibody RP1073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-12
Answer
The products we sell, including anti-TLR8 antibody RP1073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-TLR8 antibody RP1073. Let me know if you need anything else.
J. Evans
Verified customer
Asked: 2015-02-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-26
Question
Our team were happy with the WB result of your anti-TLR8 antibody. However we have been able to see positive staining in liver membrane using this antibody. Is that expected? Could you tell me where is TLR8 supposed to be expressed?
K. Taylor
Verified customer
Asked: 2014-04-30
Answer
From what I have seen in literature, liver does express TLR8. Generally TLR8 expresses in membrane. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425
Boster Scientific Support
Answered: 2014-04-30
Question
Can you help my question with product RP1073, anti-TLR8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Wu
Verified customer
Asked: 2013-11-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1073 anti-TLR8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-08
