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All lanes: Anti TLR8 (RP1073) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 120KD
Observed bind size: 120KD
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Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 120KD
Observed bind size: 120KD
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All lanes: Anti TLR8 (RP1073) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 120KD
Observed bind size: 120KD
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Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=TLR8 (Toll-like receptor 8) is a protein that in humans is encoded by the TLR8 gene. TLR8 has also been designated as CD288 (cluster of differentiation 288).The TLR8 gene is mapped to Xp22.3-p22.2 by Chuang and Ulevitch (2000) and Du et al. (2000). The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. TLR8 recognises G-rich oligonucleotides.
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Key=application_details, value=Western blot, 0.1-0.5μg/ml, Human, Rat

Key=subcellular_localization, value= Membrane ; Single-pass type I membrane protein .
Key=sequence_similarities, value=Belongs to the Toll-like receptor family.
Key=tissue_specificity, value= Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
Key=protein_function, value= Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. .
Key=application_notes, value=WB: The detection limit for TLR8 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 13213|sc 32995|sc 25467|sc 373760|sc 17585|sc 135584|sc 13212 R
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human TLR8, identical to the related mouse sequence.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
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Anti-Toll-like receptor 8 TLR8 Antibody Picoband®

TLR8 antibody

Boster Bio Anti-Toll-like receptor 8 TLR8 Antibody catalog # RP1073. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: RP1073
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Toll-like receptor 8 TLR8 Antibody Picoband®

View all TLR8 Antibodies

SKU/Catalog Number

RP1073

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Toll-like receptor 8 TLR8 Antibody catalog # RP1073. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Toll-like receptor 8 TLR8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1073)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TLR8, identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1073 is reactive to TLR8 in Human, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

120 kDa

Calculated molecular weight

119828 MW

Background of TLR8

TLR8 (Toll-like receptor 8) is a protein that in humans is encoded by the TLR8 gene. TLR8 has also been designated as CD288 (cluster of differentiation 288).The TLR8 gene is mapped to Xp22.3-p22.2 by Chuang and Ulevitch (2000) and Du et al. (2000). The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. TLR8 recognises G-rich oligonucleotides.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

RP1073 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: Rat Liver Tissue, HEPG2 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For TLR8 (Source: Uniprot.org, NCBI)

Gene Name

TLR8

Full Name

Toll-like receptor 8

Weight

119828 MW

Superfamily

Toll-like receptor family

Alternative Names

Toll-like receptor 8;CD288;TLR8;UNQ249/PRO286; TLR8 CD288 toll like receptor 8 toll-like receptor 8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TLR8, check out the TLR8 Infographic

TLR8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TLR8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-Toll-like receptor 8 TLR8 Antibody Picoband®

Question

Is this RP1073 anti-TLR8 antibody reactive to the isotypes of TLR8?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

The immunogen of RP1073 anti-TLR8 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TLR8 (881-907aa DAYISYDTKDASVTDWVINELRYHLEE), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-09

Question

I see that the anti-TLR8 antibody RP1073 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-08

Question

Is there a BSA free version of anti-TLR8 antibody RP1073 available?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TLR8 antibody RP1073 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-23

Question

Would anti-TLR8 antibody RP1073 work for WB with placenta?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

According to the expression profile of placenta, TLR8 is highly expressed in placenta. So, it is likely that anti-TLR8 antibody RP1073 will work for WB with placenta.

Boster Scientific Support

Answered: 2020-03-17

Question

Does RP1073 anti-TLR8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

You can see on the product datasheet, RP1073 anti-TLR8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-24

Question

Please see the WB image, lot number and protocol we used for placenta using anti-TLR8 antibody RP1073. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-20

Question

We are currently using anti-TLR8 antibody RP1073 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

The anti-TLR8 antibody (RP1073) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-09

Question

I am interested in using your anti-TLR8 antibody for cellular response to mechanical stimulus studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

I appreciate your inquiry. This RP1073 anti-TLR8 antibody is tested on rat liver tissue, tissue lysate, hepg2 whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-25

Question

Would anti-TLR8 antibody RP1073 work on pig WB with blood?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

Our lab technicians have not tested anti-TLR8 antibody RP1073 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TLR8 antibody RP1073 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-24

Question

I was wanting to use your anti-TLR8 antibody for WB for rat placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat placenta identification?

T. Evans

Verified customer

Asked: 2018-01-08

Answer

As indicated on the product datasheet, RP1073 anti-TLR8 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-08

Question

We have seen staining in human liver. Are there any suggestions? Is anti-TLR8 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2017-12-18

Answer

From what I have seen in literature liver does express TLR8. From what I have seen in Uniprot.org, TLR8 is expressed in blood, placenta, liver, among other tissues. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425

Boster Scientific Support

Answered: 2017-12-18

Question

Is a blocking peptide available for product anti-TLR8 antibody (RP1073)?

Verified Customer

Verified customer

Asked: 2017-08-23

Answer

We do provide the blocking peptide for product anti-TLR8 antibody (RP1073). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-08-23

Question

We need to test anti-TLR8 antibody RP1073 on rat placenta for research purposes, then I may be interested in using anti-TLR8 antibody RP1073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-12

Answer

The products we sell, including anti-TLR8 antibody RP1073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-TLR8 antibody RP1073. Let me know if you need anything else.

J. Evans

Verified customer

Asked: 2015-02-26

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-02-26

Question

Our team were happy with the WB result of your anti-TLR8 antibody. However we have been able to see positive staining in liver membrane using this antibody. Is that expected? Could you tell me where is TLR8 supposed to be expressed?

K. Taylor

Verified customer

Asked: 2014-04-30

Answer

From what I have seen in literature, liver does express TLR8. Generally TLR8 expresses in membrane. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425

Boster Scientific Support

Answered: 2014-04-30

Question

Can you help my question with product RP1073, anti-TLR8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

G. Wu

Verified customer

Asked: 2013-11-08

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1073 anti-TLR8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-11-08

Order DetailsPrice
RP1073

100μg

$370
RP1073-10ug

10μg sample (liquid)

$99
RP1073-Biotin

100 μg Biotin conjugated

$570
RP1073-Cy3

100 μg Cy3 conjugated

$570
RP1073-Dylight488

100 μg Dylight488 conjugated

$570
RP1073-Dylight550

100 μg Dylight550 conjugated

$570
RP1073-Dylight594

100 μg Dylight594 conjugated

$570
RP1073-FITC

100 μg FITC conjugated

$570
RP1073-HRP

100 μg HRP conjugated

$570
RP1073-APC

100 μg APC conjugated

$670
RP1073-PE

100 μg PE conjugated

$670
RP1073-iFluor647

100 μg iFluor647 conjugated

$670
RP1073-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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RP1073
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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