Anti-Thymidine Kinase 1/TK1 Antibody Picoband®

Product Name

Anti-Thymidine Kinase 1/TK1 Antibody Picoband®

View all Thymidine Kinase 1 Antibodies

SKU/Catalog Number

PB9832

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Thymidine Kinase 1/TK1 Antibody Picoband® catalog # PB9832. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Thymidine Kinase 1/TK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9832)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human TK1 recombinant protein (Position: S2-N234). Human TK1 shares 87.1% and 91.7% amino acid (aa) sequence identity with mouse and rat TK1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9832 is reactive to TK1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

25 kDa

Calculated molecular weight

25469 MW

Background of Thymidine Kinase 1

Thymidine kinase 1, soluble (gene name TK1), is a human thymidine kinase. Thymidine kinase catalyzes the phosphorylation of thymidine to deoxythymidine monophosphate. This gene is mapped to 17q23.2-q25.3. It is estimated that the maximal size of the TK1 gene is 14 kb and its minimal size between 4 and 5 kb. The gene contains many noncoding inserts and numerous Alu sequences. There are two forms of this protein, which have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9832 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: HELA Whole Cell, SMMC Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For TK1 (Source: Uniprot.org, NCBI)

Alternative Names

Thymidine kinase, cytosolic;2.7.1.21;TK1; TK1 TK2 thymidine kinase 1 thymidine kinase, cytosolic|cytosolic thymidine kinase|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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