Anti-TIE1 Antibody

Tie-1 antibody

Boster Bio Anti-TIE1 Antibody (Catalog # A02206). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A02206
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-TIE1 Antibody

View all Tie-1 Antibodies

SKU/Catalog Number

A02206

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TIE1 Antibody (Catalog # A02206). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TIE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02206)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human TIE1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02206 is reactive to TIE1 in Human

Applications

A02206 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

125090 MW

Background of Tie-1

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.

Partanen J., Mol. Cell. Biol. 12:1698-1707(1992).
Partanen J.M., Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases.
Zhang Z., Protein Sci. 13:2819-2824(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For TIE1 (Source: Uniprot.org, NCBI)

Gene Name

TIE1

Full Name

Tyrosine-protein kinase receptor Tie-1

Weight

125090 MW

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.10; EC 2.7.10.1; JTK14soluble TIE1 variant 3; receptor tyrosine kinase; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 5; Tie1; Tie-1; TIEsoluble TIE1 variant 4; tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; tyrosine-protein kinase receptor Tie-1 TIE1 JTK14, TIE tyrosine kinase with immunoglobulin like and EGF like domains 1 tyrosine-protein kinase receptor Tie-1|tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TIE1, check out the TIE1 Infographic

TIE1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TIE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-TIE1 Antibody

Question

My question regarding product A02206, anti-TIE1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02206 anti-TIE1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-17

Question

I was wanting to use your anti-TIE1 antibody for WB for human right lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right lung identification?

Verified Customer

Verified customer

Asked: 2019-01-24

Answer

You can see on the product datasheet, A02206 anti-TIE1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-24

Question

We are currently using anti-TIE1 antibody A02206 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-11-16

Answer

The anti-TIE1 antibody (A02206) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-16

Question

Do you have a BSA free version of anti-TIE1 antibody A02206 available?

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TIE1 antibody A02206 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-09-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lung using anti-TIE1 antibody A02206. Let me know if you need anything else.

J. Collins

Verified customer

Asked: 2017-10-05

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-05

Question

Will anti-TIE1 antibody A02206 work for WB with right lung?

T. Jackson

Verified customer

Asked: 2014-06-13

Answer

According to the expression profile of right lung, TIE1 is highly expressed in right lung. So, it is likely that anti-TIE1 antibody A02206 will work for WB with right lung.

Boster Scientific Support

Answered: 2014-06-13

Order DetailsPrice
A02206-100uL

100uL

$360

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A02206
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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