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Product Info Summary
| SKU: | A02206 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TIE1 Antibody
SKU/Catalog Number
A02206
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TIE1 Antibody (Catalog # A02206). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02206)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human TIE1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02206 is reactive to TIE1 in Human
Reconstitution
Calculated molecular weight
125090 MW
Background of Tie-1
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
Partanen J., Mol. Cell. Biol. 12:1698-1707(1992).
Partanen J.M., Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases.
Zhang Z., Protein Sci. 13:2819-2824(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02206 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells, K562 cells and Jurkat cells, using TIE1 antibody A02206.
Protein Target Info & Infographic
Gene/Protein Information For TIE1 (Source: Uniprot.org, NCBI)
Gene Name
TIE1
Full Name
Tyrosine-protein kinase receptor Tie-1
Weight
125090 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase receptor Tie-1;2.7.10.1;TIE1;TIE; TIE1 JTK14, TIE tyrosine kinase with immunoglobulin like and EGF like domains 1 tyrosine-protein kinase receptor Tie-1|tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TIE1, check out the TIE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TIE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIE1 Antibody (A02206)
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No publications found for A02206
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TIE1 Antibody
Question
My question regarding product A02206, anti-TIE1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02206 anti-TIE1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-17
Question
I was wanting to use your anti-TIE1 antibody for WB for human right lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right lung identification?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
You can see on the product datasheet, A02206 anti-TIE1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-24
Question
We are currently using anti-TIE1 antibody A02206 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
The anti-TIE1 antibody (A02206) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-16
Question
Do you have a BSA free version of anti-TIE1 antibody A02206 available?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TIE1 antibody A02206 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lung using anti-TIE1 antibody A02206. Let me know if you need anything else.
J. Collins
Verified customer
Asked: 2017-10-05
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-05
Question
Will anti-TIE1 antibody A02206 work for WB with right lung?
T. Jackson
Verified customer
Asked: 2014-06-13
Answer
According to the expression profile of right lung, TIE1 is highly expressed in right lung. So, it is likely that anti-TIE1 antibody A02206 will work for WB with right lung.
Boster Scientific Support
Answered: 2014-06-13
