Anti-Thymopoietin TMPO Antibody

Product Name

Anti-Thymopoietin TMPO Antibody

View all Thymopoietin/LAP2 Antibodies

SKU/Catalog Number

A03278-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Thymopoietin TMPO Antibody (Catalog # A03278-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Thymopoietin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Thymopoietin TMPO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03278-1)

Host

Rabbit

Contents

Thymopoietin Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Thymopoietin antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human Thymopoietin. The immunogen is located within amino acids 80 - 130 of Thymopoietin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of Thymopoietin are known to exist; this antibody will detect all isoforms. Thymopoietin antibody is predicted to not cross-react with other LEM domain-containing proteins.

Reactive Species

A03278-1 is reactive to TMPO in Human, Mouse

Applications

A03278-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

75492 MW

Background of Thymopoietin/LAP2

Thymopoietin (TMPO), also known as LAP2, is a widely expressed lem-domain-containing nuclear protein that is thought to play an important role in the regulation of nuclear architecture by binding lamin B1 and chromosomes in a manner regulated by phosphorylation during mitosis. Thymopoietin associates with nucleoplasmic lamins A/C, which are encoded by the dilated cardiomyopathy (DCM) gene LMNA; mutations in Thymopoietin disrupt this binding, suggesting that Thymopoietin may be a candidate disease gene for DCM.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Thymopoietin antibody can be used for detection of Thymopoietin by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of Thymopoietin in HeLa cell lysate with Thymopoietin antibody at 0.25 μg/mLl in (A) the absence and (B) the presence of blocking peptide.

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Immunocytochemistry of Thymopoietin in HeLa cells with Thymopoietin antibody at 2.5 μg/mL.

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Immunofluorescence of Thymopoietin in HeLa cells with Thymopoietin antibody at 5 μg/mL.

Gene/Protein Information For TMPO (Source: Uniprot.org, NCBI)

Alternative Names

CMD1T; lamina-associated polypeptide 2; LAP2PRO0868; LEM domain containing 4; LEMD4; MGC61508; Thymopoietin isoform alpha; Thymopoietin; Thymopoietin, isoforms beta/gamma; Thymopoietin-related peptide isoform alpha; Thymopoietin-related peptide isoforms beta/gamma; TMPO; TP alpha; TP beta/gamma; TP; TPRP isoform alpha; TPRP isoforms beta/gamma TMPO CMD1T, LAP2, LEMD4, PRO0868, TP thymopoietin thymopoietin|LEM domain containing 4|lamina-associated polypeptide 2

For more info on TMPO, check out the TMPO Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TMPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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