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Product Info Summary
| SKU: | A03321-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Thymidine Kinase 2/TK2 Antibody Picoband®
View all Thymidine Kinase 2 Antibodies
SKU/Catalog Number
A03321-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Thymidine Kinase 2/TK2 Antibody Picoband® catalog # A03321-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Thymidine Kinase 2/TK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03321-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Thymidine Kinase 2/TK2 recombinant protein (Position: V34-P265).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03321-1 is reactive to TK2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
31.005kDa
Background of Thymidine Kinase 2
Thymidine kinase 2, mitochondrial is a protein that in humans is encoded by the TK2 gene. This gene encodes a deoxyribonucleoside kinase that specifically phosphorylates thymidine, deoxycytidine, and deoxyuridine. The encoded enzyme localizes to the mitochondria and is required for mitochondrial DNA synthesis. Mutations in this gene are associated with a myopathic form of mitochondrial DNA depletion syndrome. Alternate splicing results in multiple transcript variants encoding distinct isoforms, some of which lack transit peptide, so are not localized to mitochondria.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03321-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human SiHa whole cell, human U-87 MG whole cell, human A549 whole cell
FCM: Jurkat cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Thymidine Kinase 2/TK2 using anti-Thymidine Kinase 2/TK2 antibody (A03321-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human U-87 MG whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thymidine Kinase 2/TK2 antigen affinity purified polyclonal antibody (Catalog # A03321-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thymidine Kinase 2/TK2 at approximately 25 kDa. The expected band size for Thymidine Kinase 2/TK2 is at 31 kDa.
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Figure 2. Flow Cytometry analysis of Jurkat cells using anti-Thymidine Kinase 2/TK2 antibody (A03321-1).
Overlay histogram showing Jurkat cells stained with A03321-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Thymidine Kinase 2/TK2 Antibody (A03321-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TK2 (Source: Uniprot.org, NCBI)
Gene Name
TK2
Full Name
Thymidine kinase 2, mitochondrial
Weight
31.005kDa
Superfamily
DCK/DGK family
Alternative Names
Glutathione S-transferase Mu 3; GST class-mu 3; GSTM3-3; hGSTM3-3; GSTM3; GST5; TK2 MTDPS2, MTTK, PEOB3, SCA31 thymidine kinase 2 thymidine kinase 2, mitochondrial|thymidine kinase 2|mitochondrial thymidine kinase|mt-TK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TK2, check out the TK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thymidine Kinase 2/TK2 Antibody Picoband® (A03321-1)
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