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Product Info Summary
| SKU: | A05024 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Thrombospondin 4 THBS4 Antibody
View all Thrombospondin-4 Antibodies
SKU/Catalog Number
A05024
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Thrombospondin 4 THBS4 Antibody catalog # A05024. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thrombospondin 4 THBS4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05024)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 551-600
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05024 is reactive to THBS4 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
105869 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05024 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For THBS4 (Source: Uniprot.org, NCBI)
Gene Name
THBS4
Full Name
Thrombospondin-4
Weight
105869 MW
Superfamily
thrombospondin family
Alternative Names
Thrombospondin-4;THBS4;TSP4; THBS4 TSP-4, TSP4 thrombospondin 4 thrombospondin-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on THBS4, check out the THBS4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THBS4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thrombospondin 4 THBS4 Antibody (A05024)
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No publications found for A05024
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Thrombospondin 4 THBS4 Antibody
Question
We are currently using anti-Thrombospondin 4 antibody A05024 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The anti-Thrombospondin 4 antibody (A05024) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for synovium using anti-Thrombospondin 4 antibody A05024. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
Do you have a BSA free version of anti-Thrombospondin 4 antibody A05024 available?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Thrombospondin 4 antibody A05024 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-10
Question
Will anti-Thrombospondin 4 antibody A05024 work for IHC with synovium?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
According to the expression profile of synovium, THBS4 is highly expressed in synovium. So, it is likely that anti-Thrombospondin 4 antibody A05024 will work for IHC with synovium.
Boster Scientific Support
Answered: 2020-02-07
Question
Is a blocking peptide available for product anti-Thrombospondin 4 antibody (A05024)?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
We do provide the blocking peptide for product anti-Thrombospondin 4 antibody (A05024). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-22
Question
I see that the anti-Thrombospondin 4 antibody A05024 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-21
Question
I was wanting to use your anti-Thrombospondin 4 antibody for IHC for human synovium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human synovium identification?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
As indicated on the product datasheet, A05024 anti-Thrombospondin 4 antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human synovium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-19
Question
Would anti-Thrombospondin 4 antibody A05024 work on horse IHC with pns?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Our lab technicians have not validated anti-Thrombospondin 4 antibody A05024 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Thrombospondin 4 antibody A05024 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse pns in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
Will A05024 anti-Thrombospondin 4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
You can see on the product datasheet, A05024 anti-Thrombospondin 4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-12
Question
We are interested in to test anti-Thrombospondin 4 antibody A05024 on human synovium for research purposes, then I may be interested in using anti-Thrombospondin 4 antibody A05024 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The products we sell, including anti-Thrombospondin 4 antibody A05024, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-17
Question
Here is the WB image, lot number and protocol we used for synovium using anti-Thrombospondin 4 antibody A05024. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-27
Question
Can you help my question with product A05024, anti-Thrombospondin 4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Moore
Verified customer
Asked: 2016-08-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05024 anti-Thrombospondin 4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-17
Question
Is this A05024 anti-Thrombospondin 4 antibody reactive to the isotypes of THBS4?
D. Miller
Verified customer
Asked: 2016-04-27
Answer
The immunogen of A05024 anti-Thrombospondin 4 antibody is Synthetic peptide from human protein at AA range: 551-600. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-04-27
