Product Info Summary
SKU: | A03253 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Thrombospondin 2 Antibody
View all Thrombospondin-2 Antibodies
SKU/Catalog Number
A03253
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Thrombospondin 2 Antibody catalog # A03253. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thrombospondin 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03253)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human THBS2 at amino acid range 1000-1050
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03253 is reactive to THBS2 in Human, Mouse, Rat
Applications
A03253 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
129991 MW
Background of Thrombospondin-2
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of mouse-heart lysis using THBS2 antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
Click image to see more details
Immunohistochemical analysis of paraffin-embedded rat-testis, antibody was diluted at 1:100
Protein Target Info & Infographic
Gene/Protein Information For THBS2 (Source: Uniprot.org, NCBI)
Gene Name
THBS2
Full Name
Thrombospondin-2
Weight
129991 MW
Superfamily
thrombospondin family
Alternative Names
THBS2; thrombospondin 2; Thrombospondin2; Thrombospondin-2; TSP-2; TSP2thrombospondin-2 THBS2 TSP2 thrombospondin 2 thrombospondin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on THBS2, check out the THBS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THBS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thrombospondin 2 Antibody (A03253)
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