Anti-THAP11 Monoclonal Antibody

Product Name

Anti-THAP11 Monoclonal Antibody

View all THAP11 Antibodies

SKU/Catalog Number

M08519

Size

100ul

Form

Liquid

Description

Boster Bio Anti-THAP11 Monoclonal Antibody catalog # M08519. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-THAP11 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08519)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

3F3

Isotype

IgG

Immunogen

Purified recombinant fragment of human THAP11 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08519 is reactive to THAP11 in Human

Applications

M08519 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

34455 MW

Background of THAP11

Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000

Validation Images & Assay Conditions

Click image to see more details

Figure 4. Flow Cytometry validation of THAP11 using Anti-THAP11 Monoclonal Antibody (M08519).

Flow cytometric (FCM) analysis of HeLa cells using THAP11 Monoclonal Antibody (green) and negative control (purple).

Click image to see more details

Figure 3. Immunofluorescent staining data of THAP11 using Anti-THAP11 Monoclonal Antibody (M08519).

Immunofluorescence (IF) analysis of NTERA-2 cells using THAP11 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Click image to see more details

Figure 2. Immunohistochemistry validation of THAP11 using Anti-THAP11 Monoclonal Antibody (M08519).

Immunohistochemistry (IHC) analysis of paraffin-embedded colon cancer tissues (left) and ovary cancer tissues (right) with DAB staining using THAP11 Monoclonal Antibody.
For more protocol information of IHC

Click image to see more details

Figure 1. Western blotting validation for Anti-THAP11 Monoclonal Antibody M08519

Western Blot (WB) analysis using THAP11 Monoclonal Antibody against HeLa (1) and NTERA-2 (2) cell lysate.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For THAP11 (Source: Uniprot.org, NCBI)

Alternative Names

CTG-B43a; CTG-B45d; HRIHFB2206; Ronin; THAP domain containing 11; THAP domain-containing protein 11; THAP11 THAP11 CTG-B43a, CTG-B45d, HRIHFB2206, RONIN THAP domain containing 11 THAP domain-containing protein 11

For more info on THAP11, check out the THAP11 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for THAP11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the THAP11 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis