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Product Info Summary
| SKU: | A00892 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-TGF beta2 TGFB2 Antibody
View all TGF-beta 2 Antibodies
SKU/Catalog Number
A00892
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TGF beta2 TGFB2 Antibody catalog # A00892. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TGF beta2 TGFB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00892)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 61-110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00892 is reactive to TGFB2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
47748 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00892 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For TGFB2 (Source: Uniprot.org, NCBI)
Gene Name
TGFB2
Full Name
Transforming growth factor beta-2 proprotein
Weight
47748 MW
Superfamily
TGF-beta family
Alternative Names
Transforming growth factor beta-2;TGF-beta-2;BSC-1 cell growth inhibitor;Cetermin;Glioblastoma-derived T-cell suppressor factor;G-TSF;Polyergin;Latency-associated peptide;LAP;TGFB2; TGFB2 G-TSF, LDS4, TGF-beta2 transforming growth factor beta 2 transforming growth factor beta-2 proprotein|BSC-1 cell growth inhibitor|cetermin|glioblastoma-derived T-cell suppressor factor|polyergin|prepro-transforming growth factor beta-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TGFB2, check out the TGFB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TGFB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TGF beta2 TGFB2 Antibody (A00892)
Hello CJ!
A00892 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Potential Mechanisms Underlying the Hepatic–Protective Effects of Danshensu on Iron Overload Mice
Tao YJ,Chen Q,Wang L,Yang X,Cun Q,Yang WY,Zhong H.Pirfenidone suppresses the abnormal activation of human Müller cells after platelet-derived growth factor-BB stimulation.Int J Ophthalmol.2019 Jul 18;12(7):1075-1082.doi:10.18240/ijo.2019.07.04.PMID:31341795;PMCID:PMC6629804.
Species: Human
A00892 usage in article: APP:WB, SAMPLE:MULLER CELL, DILUTION:NA
[HTML] Pirfenidone suppresses the abnormal activation of human Müller cells after platelet-derived growth factor-BB stimulation Yi-Jin Tao, et al. Int J Ophthalmol. 2019; 12(7): 1075–1082. Published online 2019 Jul 18. doi: 10.18240/ijo.2019.07.04
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-TGF beta2 TGFB2 Antibody
Question
We are currently using anti-TGF beta2 antibody A00892 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The anti-TGF beta2 antibody (A00892) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-29
Question
Is there a BSA free version of anti-TGF beta2 antibody A00892 available?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TGF beta2 antibody A00892 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-27
Question
I have attached the WB image, lot number and protocol we used for lung using anti-TGF beta2 antibody A00892. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-21
Question
We have seen staining in mouse lung. Any tips? Is anti-TGF beta2 antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
From literature lung does express TGFB2. From Uniprot.org, TGFB2 is expressed in lung, thalamus, among other tissues. Regarding which tissues have TGFB2 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 1764261
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-04
Question
My team were well pleased with the WB result of your anti-TGF beta2 antibody. However we have observed positive staining in thalamus latency-associated peptide: secreted, using this antibody. Is that expected? Could you tell me where is TGFB2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
From what I have seen in literature, thalamus does express TGFB2. Generally TGFB2 expresses in latency-associated peptide: secreted,, transforming growth factor beta-2: secreted. Regarding which tissues have TGFB2 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 1764261
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-18
Question
Does anti-TGF beta2 antibody A00892 work for IHC with lung?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
According to the expression profile of lung, TGFB2 is highly expressed in lung. So, it is likely that anti-TGF beta2 antibody A00892 will work for IHC with lung.
Boster Scientific Support
Answered: 2020-02-17
Question
Can you help my question with product A00892, anti-TGF beta2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Miller
Verified customer
Asked: 2020-02-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00892 anti-TGF beta2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-11
Question
Does A00892 anti-TGF beta2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
It shows on the product datasheet, A00892 anti-TGF beta2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-04
Question
I was wanting to use your anti-TGF beta2 antibody for IHC for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
It shows on the product datasheet, A00892 anti-TGF beta2 antibody has been tested for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-19
Question
Is this A00892 anti-TGF beta2 antibody reactive to the isotypes of TGFB2?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
The immunogen of A00892 anti-TGF beta2 antibody is Synthetic peptide from human protein at AA range: 61-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-07
Question
I see that the anti-TGF beta2 antibody A00892 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-26
Question
Is a blocking peptide available for product anti-TGF beta2 antibody (A00892)?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
We do provide the blocking peptide for product anti-TGF beta2 antibody (A00892). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-02
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-TGF beta2 antibody A00892. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-03
Question
My question regards to test anti-TGF beta2 antibody A00892 on mouse lung for research purposes, then I may be interested in using anti-TGF beta2 antibody A00892 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Kulkarni
Verified customer
Asked: 2016-05-09
Answer
The products we sell, including anti-TGF beta2 antibody A00892, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-09
