Product Info Summary
SKU: | A00803-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-TBX5 Antibody Picoband™
SKU/Catalog Number
A00803-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TBX5 Antibody Picoband™ catalog # A00803-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TBX5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00803-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TBX5, which shares 82.8% and 86.2% amino acid (aa) sequence identity with mouse and rat TBX5, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00803-2 is reactive to TBX5 in Human, Mouse, Rat
Applications
A00803-2 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
58 kDa
Calculated molecular weight
22300 MW
Background of TBX5
T-box transcription factor TBX5 is a protein that in humans is encoded by the TBX5 gene. It is mapped to 12q24.21. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For TBX5 (Source: Uniprot.org, NCBI)
Gene Name
TBX5
Full Name
T-box transcription factor TBX5
Weight
22300 MW
Alternative Names
HOS; T-box 5; T-box protein 5; T-box transcription factor TBX5; TBX5 TBX5 HOS T-box transcription factor 5 T-box transcription factor TBX5|T-box 5|T-box protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TBX5, check out the TBX5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TBX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TBX5 Antibody Picoband™ (A00803-2)
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No publications found for A00803-2
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TBX5 Antibody Picoband™
Question
Does anti-TBX5 antibody A00803-2 work for WB with lung spleen?
Z. Krishna
Verified customer
Asked: 2019-11-04
Answer
According to the expression profile of lung spleen, TBX5 is highly expressed in lung spleen. So, it is likely that anti-TBX5 antibody A00803-2 will work for WB with lung spleen.
Boster Scientific Support
Answered: 2019-11-04
Question
We are currently using anti-TBX5 antibody A00803-2 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
W. Krishna
Verified customer
Asked: 2019-08-01
Answer
The anti-TBX5 antibody (A00803-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-01
Question
Can you help my question with product A00803-2, anti-TBX5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Baker
Verified customer
Asked: 2015-01-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00803-2 anti-TBX5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-01-21
Question
I see that the anti-TBX5 antibody A00803-2 works with WB, what is the protocol used to produce the result images on the product page?
E. Anderson
Verified customer
Asked: 2013-09-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-13
Question
Is a blocking peptide available for product anti-TBX5 antibody (A00803-2)?
A. Mangal
Verified customer
Asked: 2013-02-28
Answer
We do provide the blocking peptide for product anti-TBX5 antibody (A00803-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-02-28
Question
I have attached the WB image, lot number and protocol we used for lung spleen using anti-TBX5 antibody A00803-2. Please let me know if you require anything else.
S. Jackson
Verified customer
Asked: 2013-02-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-05