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Product Info Summary
| SKU: | A02672 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TAZ Antibody
SKU/Catalog Number
A02672
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TAZ Antibody (Catalog # A02672). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAZ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02672)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.386~390 (V-E-S-A-L) derived from Human TAZ.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02672 is reactive to WWTR1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
28530 MW
Background of TAZ/WWTR1
Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
Kanai F., Marignani P.A., Sarbassova D., Yagi R. EMBO J. 19:6778-6791(2000)
Lei Q.Y., Zhang H., Zhao B., Zha Z.Y. Mol. Cell. Biol. 28:2426-2436(2008)
Varelas X., Sakuma R., Samavarchi-Tehrani P., Peerani R. Nat. Cell Biol. 10:837-848(2008)
Di Palma T., D'Andrea B., Liguori G.L., Liguoro A. Exp. Cell Res. 315:162-175(2009)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02672 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 49kd
Western blotting: 1:500~1:1000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of WWTR1 using anti-WWTR1 antibody (A02672).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WWTR1 antigen affinity purified polyclonal antibody (Catalog # A02672) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for WWTR1.
Protein Target Info & Infographic
Gene/Protein Information For WWTR1 (Source: Uniprot.org, NCBI)
Gene Name
WWTR1
Full Name
WW domain-containing transcription regulator protein 1
Weight
28530 MW
Alternative Names
Inhibitor of growth protein 4;p29ING4;ING4;My036; WWTR1 TAZ WW domain containing transcription regulator 1 WW domain-containing transcription regulator protein 1|transcriptional co-activator with PDZ-binding motif
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WWTR1, check out the WWTR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WWTR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAZ Antibody (A02672)
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No publications found for A02672
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Customer Q&As
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1 Customer Q&As for Anti-TAZ Antibody
Question
We are currently using anti-TAZ antibody A02672 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-TAZ antibody (A02672) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
