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Product Info Summary
| SKU: | A04714 |
|---|---|
| Size: | 200μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Tapasin TAPBP Antibody
SKU/Catalog Number
A04714
Size
200μg/vial
Form
Liquid
Description
Boster Bio Anti-Tapasin TAPBP Antibody (Catalog# A04714). Tested in IP, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tapasin TAPBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04714)
Host
Rabbit
Contents
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide corresponding to aa 2-20 of human tapasin with a cysteine residue added
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Weakly cross-reacts with bovine Tapasin.
Reactive Species
A04714 is reactive to TAPBP in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
47626 MW
Background of Tapasin
EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz (2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence (GRCh37). Ernst et al. (1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al. (1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04714 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunoprecipitation (1:100)
Western Blot (1:1,000, colorimetric and ECL). Detects a band of ~48kDa.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Western blot analysis of Tapasin expression in MW marker (lane 1) and A431 whole cell lysates (lane 2). Tapasinat 48KD was detected using rabbit anti-Tapasin Antigen Affinity purified polyclonal antibody (Catalog # A04714) at 1: 1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For TAPBP (Source: Uniprot.org, NCBI)
Gene Name
TAPBP
Full Name
Tapasin
Weight
47626 MW
Alternative Names
Tapasin;TPN;TPSN;NGS-17;TAP-associated protein;TAP-binding protein;TAPBP;NGS17, TAPA; TAPBP NGS17, TAPA, TPN, TPSN TAP binding protein tapasin|NGS-17|TAP binding protein (tapasin)|TAP-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAPBP, check out the TAPBP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAPBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tapasin TAPBP Antibody (A04714)
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No publications found for A04714
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Tapasin TAPBP Antibody
Question
Do you have a BSA free version of anti-Tapasin antibody A04714 available?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Tapasin antibody A04714 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-31
Question
Would A04714 anti-Tapasin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Mitchell
Verified customer
Asked: 2020-02-21
Answer
You can see on the product datasheet, A04714 anti-Tapasin antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-Tapasin antibody A04714 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The anti-Tapasin antibody (A04714) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-17
Question
I have attached the WB image, lot number and protocol we used for prostate using anti-Tapasin antibody A04714. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate using anti-Tapasin antibody A04714. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-01
Question
I see that the anti-Tapasin antibody A04714 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-29
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-29
Question
Is a blocking peptide available for product anti-Tapasin antibody (A04714)?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
We do provide the blocking peptide for product anti-Tapasin antibody (A04714). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-06
Question
Will anti-Tapasin antibody A04714 work for IP with prostate?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
According to the expression profile of prostate, TAPBP is highly expressed in prostate. So, it is likely that anti-Tapasin antibody A04714 will work for IP with prostate.
Boster Scientific Support
Answered: 2018-04-26
Question
Can you help my question with product A04714, anti-Tapasin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Kulkarni
Verified customer
Asked: 2018-03-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04714 anti-Tapasin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-08
Question
Is this A04714 anti-Tapasin antibody reactive to the isotypes of TAPBP?
C. Zhao
Verified customer
Asked: 2017-12-07
Answer
The immunogen of A04714 anti-Tapasin antibody is Synthetic peptide corresponding to aa 2-20 of human tapasin with a cysteine residue added. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-07
Question
Would anti-Tapasin antibody A04714 work on horse IP with liver?
T. Wu
Verified customer
Asked: 2016-08-26
Answer
Our lab technicians have not tested anti-Tapasin antibody A04714 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Tapasin antibody A04714 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-08-26
Question
I am interested in to test anti-Tapasin antibody A04714 on human prostate for research purposes, then I may be interested in using anti-Tapasin antibody A04714 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Li
Verified customer
Asked: 2014-12-23
Answer
The products we sell, including anti-Tapasin antibody A04714, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-12-23
Question
I was wanting to use your anti-Tapasin antibody for IP for human prostate on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human prostate identification?
R. Parker
Verified customer
Asked: 2014-01-07
Answer
As indicated on the product datasheet, A04714 anti-Tapasin antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-07
