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Product Info Summary
| SKU: | A10219 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-60S ribosomal protein L39 RPL39 Antibody
SKU/Catalog Number
A10219
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-60S ribosomal protein L39 RPL39 Antibody (Catalog # A10219). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-60S ribosomal protein L39 RPL39 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10219)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RPL39.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10219 is reactive to RPL39 in Human, Mouse, Rat
Applications
A10219 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
6407 MW
Background of RPL39
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the S39E family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein is the smallest, and one of the most basic, proteins of the ribosome. This gene is co-transcribed with the U69 small nucleolar RNA gene, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Tsui S.K.W., Biochem. Mol. Biol. Int. 40:611-616(1996).
Otsuka S., Biochim. Biophys. Acta 1308:119-121(1996).
Yoshihama M., Genome Res. 12:379-390(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells and COLO cells, using RPL39 antibody A10219.
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Immunofluorescence analysis of A549 cells, using RPL39 antibody A10219.
Protein Target Info & Infographic
Gene/Protein Information For RPL39 (Source: Uniprot.org, NCBI)
Gene Name
RPL39
Full Name
60S ribosomal protein L39
Weight
6407 MW
Superfamily
eukaryotic ribosomal protein eL39 family
Alternative Names
ribosomal protein L39,60S ribosomal protein L39 RPL39 L39P42, RPL39_23_1806, RPL39 ribosomal protein L39 60S ribosomal protein L39|large ribosomal subunit protein eL39
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL39, check out the RPL39 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL39: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-60S ribosomal protein L39 RPL39 Antibody (A10219)
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No publications found for A10219
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-60S ribosomal protein L39 RPL39 Antibody
Question
Will A10219 anti-RPL39 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
You can see on the product datasheet, A10219 anti-RPL39 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-07
Question
Is a blocking peptide available for product anti-RPL39 antibody (A10219)?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
We do provide the blocking peptide for product anti-RPL39 antibody (A10219). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-25
Question
We are currently using anti-RPL39 antibody A10219 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The anti-RPL39 antibody (A10219) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-03
Question
I have attached the WB image, lot number and protocol we used for female gonad using anti-RPL39 antibody A10219. Please let me know if you require anything else.
R. Collins
Verified customer
Asked: 2018-06-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-04
Question
Do you have a BSA free version of anti-RPL39 antibody A10219 available?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RPL39 antibody A10219 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-17
Question
Can you help my question with product A10219, anti-RPL39 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10219 anti-RPL39 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for female gonad using anti-RPL39 antibody A10219. Let me know if you need anything else.
K. Bhatt
Verified customer
Asked: 2015-01-21
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-21
