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Product Info Summary
| SKU: | PB9961 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Talin 2/TLN2 Antibody Picoband®
SKU/Catalog Number
PB9961
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Talin 2/TLN2 Antibody Picoband® catalog # PB9961. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Talin 2/TLN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9961)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Talin 2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9961 is reactive to TLN2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
271 kDa
Calculated molecular weight
271613 MW
Background of Talin2
Talin 2 is a protein in humans that is encoded by the TLN2 gene. It belongs to the talin protein family. This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. Talin-2 is expressed at high levels in cardiac muscle and functions to provide linkages between the extracellular matrix and actin cytoskeleton at costamere structures to transduce force laterally.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9961 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse cardiac muscle tissue, SMMC7721 whole cell
IHC: mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Talin 2 using anti-Talin 2 antibody (PB9961).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse cardiac muscle tissue lysates,
Lane 3: SMMC7721 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Talin 2 antigen affinity purified polyclonal antibody (Catalog # PB9961) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Talin 2 at approximately 271 kDa. The expected band size for Talin 2 is at 272 kDa.
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Figure 2. IHC analysis of Talin 2 using anti-Talin 2 antibody (PB9961).
Talin 2 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Talin 2 Antibody (PB9961) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Talin 2 using anti-Talin 2 antibody (PB9961).
Talin 2 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Talin 2 Antibody (PB9961) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Talin 2 using anti-Talin 2 antibody (PB9961).
Talin 2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Talin 2 Antibody (PB9961) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TLN2 (Source: Uniprot.org, NCBI)
Gene Name
TLN2
Full Name
Talin-2
Weight
271613 MW
Alternative Names
Talin-2;TLN2;KIAA0320; Tln2|5730421P04Rik, AI507121, AI787438, AL118320, mKIAA0320|talin 2|talin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLN2, check out the TLN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Talin 2/TLN2 Antibody Picoband® (PB9961)
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Customer Q&As
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7 Customer Q&As for Anti-Talin 2/TLN2 Antibody Picoband®
Question
Is there a BSA free version of anti-Talin 2/TLN2 antibody PB9961 available?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Talin 2/TLN2 antibody PB9961 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-16
Question
Please see the WB image, lot number and protocol we used for liver using anti-Talin 2/TLN2 antibody PB9961. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-25
Question
Will anti-Talin 2/TLN2 antibody PB9961 work for IHC-P with liver?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
According to the expression profile of liver, TLN2 is highly expressed in liver. So, it is likely that anti-Talin 2/TLN2 antibody PB9961 will work for IHC-P with liver.
Boster Scientific Support
Answered: 2019-08-08
Question
We are currently using anti-Talin 2/TLN2 antibody PB9961 for mouse tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
K. Carter
Verified customer
Asked: 2019-03-20
Answer
The anti-Talin 2/TLN2 antibody (PB9961) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-20
Question
Does PB9961 anti-Talin 2/TLN2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-08
Answer
It shows on the product datasheet, PB9961 anti-Talin 2/TLN2 antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-08
Question
I was wanting to use your anti-Talin 2/TLN2 antibody for IHC-P for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
You can see on the product datasheet, PB9961 anti-Talin 2/TLN2 antibody has been tested for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-29
Question
Does anti-Talin 2/TLN2 antibody PB9961 work on dog WB with cervix carcinoma?
G. Mitchell
Verified customer
Asked: 2018-05-09
Answer
Our lab technicians have not tested anti-Talin 2/TLN2 antibody PB9961 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Talin 2/TLN2 antibody PB9961 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-09
