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Product Info Summary
| SKU: | A00944-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-TAL1 Antibody Picoband®
SKU/Catalog Number
A00944-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TAL1 Antibody Picoband® catalog # A00944-4. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TAL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00944-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TAL1, which shares 82.4% amino acid (aa) sequence identity with mouse and rat TAL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00944-4 is reactive to TAL1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
40-45 kDa
Calculated molecular weight
50408 MW
Background of SCL/Tal1
T-cell acute lymphocytic leukemia protein 1 is a protein that in humans is encoded by the TAL1 gene. The protein encoded by TAL1 is a basic helix-loop-helix transcription factor. TAL1 is a transcriptionally complex gene that is expressed throughout development, activating or repressing transcription in hematopoietic, neural, and endothelial precursors. This gene is mapped to chromosome 1p33.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00944-4 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Positive Control
WB: human Jurkat whole cell, human HEL whole cell, human MOLT-4 whole cell, rat lung tissue, rat spleen tissue, mouse lung tissue, mouse spleen tissue
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TAL1 using anti-TAL1 antibody (A00944-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: rat lung tissue lysate,
Lane 5: rat spleen tissue lysate,
Lane 6: mouse lung tissue lysate,
Lane 7: mouse spleen tissue lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAL1 antigen affinity purified polyclonal antibody (Catalog # A00944-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAL1 at approximately 40-45 kDa. The expected band size for TAL1 is at 34 kDa.
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Figure 2. Flow Cytometry analysis of HEL cells using anti-TAL1 antibody (A00944-4).
Overlay histogram showing HEL cells stained with A00944-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAL1 Antibody (A00944-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TAL1 (Source: Uniprot.org, NCBI)
Gene Name
TAL1
Full Name
T-cell acute lymphocytic leukemia protein 1
Weight
50408 MW
Alternative Names
T-cell acute lymphocytic leukemia protein 1; TAL-1; Class A basic helix-loop-helix protein 17; bHLHa17; Stem cell protein; T-cell leukemia/lymphoma protein 5; TAL1; BHLHA17; SCL; TCL5 TAL1 SCL, TCL5, bHLHa17, tal-1 TAL bHLH transcription factor 1, erythroid differentiation factor T-cell acute lymphocytic leukemia protein 1|T-cell acute lymphocytic leukemia 1|T-cell leukemia/lymphoma protein 5|class A basic helix-loop-helix protein 17|stem cell protein|tal-1 product
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAL1, check out the TAL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAL1 Antibody Picoband® (A00944-4)
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