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Product Info Summary
| SKU: | M09403 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-TAGAP Rabbit Monoclonal Antibody
SKU/Catalog Number
M09403
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TAGAP Rabbit Monoclonal Antibody catalog # M09403. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAGAP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09403)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
30T40
Isotype
IgG
Immunogen
A synthesized peptide derived from human TAGAP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09403 is reactive to TAGAP in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
72 kDa
Calculated molecular weight
26132 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09403 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: human Jurkat whole cell, human Daudi whole cell, human HL-60 whole cell, rat PC-12 whole cell, mouse thymus tissue, mouse SP2/0 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TAGAP using anti-TAGAP antibody (M09403).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGAP antigen affinity purified monoclonal antibody (Catalog # M09403) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAGAP at approximately 72 kDa. The expected band size for TAGAP is at 81 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TAGAP (Source: Uniprot.org, NCBI)
Gene Name
TAGAP
Full Name
T-cell activation Rho GTPase-activating protein
Weight
26132 MW
Alternative Names
Vesicle transport protein SEC20;BCL2/adenovirus E1B 19 kDa protein-interacting protein 1;Transformation-related gene 8 protein;TRG-8;BNIP1;NIP1, SEC20L;TRG8; Tagap|2610315E15Rik, TR, TRD, Tcd-1, Tcd1, Tcd1a, tcs, tcs-, tcs-1, tcs1|T cell activation Rho GTPase activating protein|T-cell activation Rho GTPase-activating protein|T-cell activation GTPase-activating protein 1|t-complex distorter 1A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAGAP, check out the TAGAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAGAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAGAP Rabbit Monoclonal Antibody (M09403)
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