Anti-TAF5 Antibody

Product Name

Anti-TAF5 Antibody

View all TAF5 Antibodies

SKU/Catalog Number

A08618

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TAF5 Antibody (Catalog # A08618). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TAF5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08618)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human TAF5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08618 is reactive to TAF5 in Human, Mouse

Reconstitution

Calculated molecular weight

86830 MW

Background of TAF5

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed.

Dubrovskaya V., EMBO J. 15:3702-3712(1996).
Tanese N., Proc. Natl. Acad. Sci. U.S.A. 93:13611-13616(1996).
Tao Y., J. Biol. Chem. 272:6714-6721(1997).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08618 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For TAF5 (Source: Uniprot.org, NCBI)

Alternative Names

Transcription initiation factor TFIID subunit 5;Transcription initiation factor TFIID 100 kDa subunit;TAF (II)100;TAFII-100;TAFII100;TAF5;TAF2D; TAF5 TAF(II)100, TAF2D, TAFII-100, TAFII100 TATA-box binding protein associated factor 5 transcription initiation factor TFIID subunit 5|TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa|TATA box binding protein (TBP)-associated factor 2D|TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD|transcription initiation factor TFIID 100 kD subunit|transcription initiation factor TFIID 100 kDa subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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