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Product Info Summary
| SKU: | PB9446 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Neurokinin 1 Receptor/TACR1 Antibody Picoband®
SKU/Catalog Number
PB9446
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Neurokinin 1 Receptor/TACR1 Antibody Picoband® catalog # PB9446. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neurokinin 1 Receptor/TACR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9446)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TACR1, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9446 is reactive to TACR1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
46251 MW
Background of NK1R
The tachykinin receptor 1 (TACR1), also known as neurokinin 1 receptor (NK1R) or substance P receptor (SPR), is a G protein coupled receptor found in the central nervous system and peripheral nervous system. The endogenous ligand for this receptor is Substance P, although it has some affinity for other tachykinins. The protein is the product of the TACR1 gene. Tachykinin receptor 1 consists of 407 amino acid residues, and it has a molecular weight of 58.000. Tachykinin receptor 1, as well as the other tachykinin receptors, is made of seven hydrophobic transmembrane (TM) domains with three extracellular and three intracellular loops, an amino-terminus and a cytoplasmic carboxy-terminus. The loops have functional sites, including two cysteines amino acids for a disulfide bridge, Asp-Arg-Tyr, which is responsible for association with arrestin and, Lys/Arg-Lys/Arg-X-X-Lys/Arg, which interacts with G-proteins.The tachykinin receptor 1 can be found in both the central and peripheral nervous system. It is present in neurons, brainstem, vascular endothelial cells, muscle, gastrointestinal tracts, genitourinary tract, pulmonary tissue, thyroid gland and different types of immune cells. The binding of SP to the tachykinin receptor 1 has been associated with the transmission of stress signals and pain, the contraction of smooth muscles and inflammation. Tachykinin receptor 1 antagonists have also been studied in migraine, emesis and psychiatric disorders. In fact, aprepitant has been proved effective in a number of pathophysiological models of anxiety and depression. Other diseases in which the tachykinin receptor 1 system is involved include asthma, rheumatoid arthritis and gastrointestinal disorders.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9446 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Human Placenta Tissue, Rat Lung Tissue, Rat Brain Tissue, U87 Whole Cell, A431 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TACR1 using anti-TACR1 antibody (PB9446).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Human Placenta Tissue Lysate at 50ug,
Lane 2: Rat Lung Tissue Lysate at 50ug,
Lane 3: Rat Brain Tissue Lysate at 50ug,
Lane 4: U87 Whole Cell Lysate at 40ug,
Lane 5: A431 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACR1 antigen affinity purified polyclonal antibody (Catalog # PB9446) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACR1 at approximately 55 kDa. The expected band size for TACR1 is at 46 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TACR1 (Source: Uniprot.org, NCBI)
Gene Name
TACR1
Full Name
Substance-P receptor
Weight
46251 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Substance-P receptor;SPR;NK-1 receptor;NK-1R;Tachykinin receptor 1;TACR1;NK1R, TAC1R; TACR1 NK1R, NKIR, SPR, TAC1R tachykinin receptor 1 substance-P receptor|NK-1 receptor|NK-1R|tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TACR1, check out the TACR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TACR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neurokinin 1 Receptor/TACR1 Antibody Picoband® (PB9446)
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No publications found for PB9446
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Customer Q&As
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1 Customer Q&As for Anti-Neurokinin 1 Receptor/TACR1 Antibody Picoband®
Question
We are currently using anti-Neurokinin 1 Receptor/TACR1 antibody PB9446 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
The anti-Neurokinin 1 Receptor/TACR1 antibody (PB9446) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-16
