Product Info Summary
SKU: | M05084 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-TAB3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05084
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TAB3 Rabbit Monoclonal Antibody catalog # M05084. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAB3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05084)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22T62
Isotype
IgG
Immunogen
A synthesized peptide derived from human TAB3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05084 is reactive to Tab3 in Human, Mouse
Applications
M05084 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
84100 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF
1:50-1:200
Validation Images & Assay Conditions
![m05084 tab3 primary antibodies wb testing 1 m05084 tab3 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m05084-tab3-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of TAB3 using anti-TAB3 antibody (M05084).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: mouse Raw264.7 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB3 antigen affinity purified monoclonal antibody (Catalog # M05084) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB3 at approximately 90 kDa. The expected band size for TAB3 is at 79 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Tab3 (Source: Uniprot.org, NCBI)
Gene Name
Tab3
Full Name
TGF-beta-activated kinase 1 and MAP3K7-binding protein 3
Weight
84100 MW
Alternative Names
CG5330; ; dNap; dNap1; dNAP-1; MAP3K7IP3; MGC45404; mitogen-activated protein kinase kinase kinase 7 interacting protein 3; Mitogen-activated protein kinase kinase kinase 7-interacting protein 3; NAP1; Nap-1; NF-kappa-B-activating protein 1; NFkB activating protein 1; p56/dCAF-4; TAB-3; TAK1-binding protein 3; TGF-beta activated kinase 1/MAP3K7 binding protein 3; TGF-beta-activated kinase 1 and MAP3K7-binding protein 3; TGF-beta-activated kinase 1-binding protein 3 Tab3|4921526G09Rik, Map3k, Map3k7ip3, mKIAA4135|TGF-beta activated kinase 1/MAP3K7 binding protein 3|TGF-beta-activated kinase 1 and MAP3K7-binding protein 3|TAK1-binding protein 3|TGF-beta-activated kinase 1-binding protein 3|mitogen-activated protein kinase kinase kinase 7-interacting protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tab3, check out the Tab3 Infographic
![Tab3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tab3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAB3 Rabbit Monoclonal Antibody (M05084)
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