Product Info Summary
SKU: | A05084-3 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TAB3 Antibody Picoband™
SKU/Catalog Number
A05084-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TAB3 Antibody Picoband™ catalog # A05084-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TAB3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05084-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TAB3 recombinant protein (Position: R70-D656).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05084-3 is reactive to Tab3 in Human
Applications
A05084-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
39125 MW
Background of NAP1
The product of this gene functions in the NF-kappaB signal transduction pathway. The encoded protein, and the similar and functionally redundant protein MAP3K7IP2/TAB2, forms a ternary complex with the protein kinase MAP3K7/TAK1 and either TRAF2 or TRAF6 in response to stimulation with the pro-inflammatory cytokines TNF or IL-1. Subsequent MAP3K7/TAK1 kinase activity triggers a signaling cascade leading to activation of the NF-kappaB transcription factor. The human genome contains a related pseudogene. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a05084 3 tab3 primary antibodies wb testing 1 a05084 3 tab3 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a05084-3-tab3-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of TAB3 using anti-TAB3 antibody (A05084-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB3 antigen affinity purified polyclonal antibody (Catalog # A05084-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB3 at approximately 90 kDa. The expected band size for TAB3 is at 79 kDa.
![a05084 3 tab3 primary antibodies fcm testing 2 a05084 3 tab3 primary antibodies fcm testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a05084-3-tab3-primary-antibodies-fcm-testing-2.jpg)
Click image to see more details
Figure 2. Flow Cytometry analysis of HL-60 cells using anti-TAB3 antibody (A05084-3).
Overlay histogram showing HL-60 cells stained with A05084-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAB3 Antibody (A05084-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a05084 3 tab3 primary antibodies fcm testing 3 a05084 3 tab3 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a05084-3-tab3-primary-antibodies-fcm-testing-3.jpg)
Click image to see more details
Figure 3. Flow Cytometry analysis of HL-60 cells using anti-TAB3 antibody (A05084-3).
Overlay histogram showing HL-60 cells stained with A05084-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAB3 Antibody (A05084-3, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Tab3 (Source: Uniprot.org, NCBI)
Gene Name
Tab3
Full Name
TGF-beta-activated kinase 1 and MAP3K7-binding protein 3
Weight
39125 MW
Alternative Names
CG5330; ; dNap; dNap1; dNAP-1; MAP3K7IP3; MGC45404; mitogen-activated protein kinase kinase kinase 7 interacting protein 3; Mitogen-activated protein kinase kinase kinase 7-interacting protein 3; NAP1; Nap-1; NF-kappa-B-activating protein 1; NFkB activating protein 1; p56/dCAF-4; TAB-3; TAK1-binding protein 3; TGF-beta activated kinase 1/MAP3K7 binding protein 3; TGF-beta-activated kinase 1 and MAP3K7-binding protein 3; TGF-beta-activated kinase 1-binding protein 3 Tab3|4921526G09Rik, Map3k, Map3k7ip3, mKIAA4135|TGF-beta activated kinase 1/MAP3K7 binding protein 3|TGF-beta-activated kinase 1 and MAP3K7-binding protein 3|TAK1-binding protein 3|TGF-beta-activated kinase 1-binding protein 3|mitogen-activated protein kinase kinase kinase 7-interacting protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tab3, check out the Tab3 Infographic
![Tab3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tab3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAB3 Antibody Picoband™ (A05084-3)
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