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Product Info Summary
| SKU: | A12428 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-T2R8 TAS2R8 Antibody
SKU/Catalog Number
A12428
Size
100μl
Form
Liquid
Description
Boster Bio Anti-T2R8 TAS2R8 Antibody catalog # A12428. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-T2R8 TAS2R8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12428)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TAS2R8. AA range:45-94
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12428 is reactive to TAS2R8 in Human
Reconstitution
Calculated molecular weight
35877 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12428 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using T2R8 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For TAS2R8 (Source: Uniprot.org, NCBI)
Gene Name
TAS2R8
Full Name
Taste receptor type 2 member 8
Weight
35877 MW
Superfamily
G-protein coupled receptor T2R family
Alternative Names
Taste receptor type 2 member 8;T2R8;Taste receptor family B member 5;TRB5;TAS2R8; TAS2R8 T2R8, TRB5 taste 2 receptor member 8 taste receptor type 2 member 8|taste receptor, family B, member 5|taste receptor, type 2, member 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAS2R8, check out the TAS2R8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAS2R8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-T2R8 TAS2R8 Antibody (A12428)
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No publications found for A12428
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Customer Q&As
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3 Customer Q&As for Anti-T2R8 TAS2R8 Antibody
Question
We are currently using anti-T2R8 antibody A12428 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The anti-T2R8 antibody (A12428) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-13
Question
Does A12428 anti-T2R8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
You can see on the product datasheet, A12428 anti-T2R8 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-10
Question
My question regarding product A12428, anti-T2R8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12428 anti-T2R8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-06
