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Product Info Summary
| SKU: | A11249-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SYNJ2BP Antibody Picoband®
View all SYNJ2BP/ARIP2 Antibodies
SKU/Catalog Number
A11249-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SYNJ2BP Antibody Picoband® catalog # A11249-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SYNJ2BP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11249-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human SYNJ2BP recombinant protein (Position: D32-L145). Human SYNJ2BP shares 88.6% and 86.8% amino acid (aa) sequence identity with mouse and rat SYNJ2BP, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11249-1 is reactive to SYNJ2BP in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
16 kDa
Calculated molecular weight
16 kDa
Background of SYNJ2BP/ARIP2
Synaptojanin-2-binding protein is a protein that in humans is encoded by the SYNJ2BP gene. SYNJ2BP, also known as Synaptojanin 2 Binding Protein, is a protein that interacts with synaptojanin 2, a phosphoinositide phosphatase found in synaptic vesicles. Synaptojanin 2 plays a crucial role in neurotransmitter release and synaptic transmission in neurons, and SYNJ2BP is implicated in regulating these processes through its interaction with synaptojanin 2. The precise molecular and cellular mechanisms of SYNJ2BP are still under investigation, with research focusing on its involvement in neural development, functional abnormalities, and neurologic disorders.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11249-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human RT4 whole cell, human MCF-7 whole cell, human PC-3 whole cell, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SYNJ2BP using anti-SYNJ2BP antibody (A11249-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNJ2BP antigen affinity purified polyclonal antibody (Catalog # A11249-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYNJ2BP at approximately 16 kDa. The expected band size for SYNJ2BP is at 16 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SYNJ2BP (Source: Uniprot.org, NCBI)
Gene Name
SYNJ2BP
Full Name
Synaptojanin-2-binding protein
Weight
16 kDa
Alternative Names
activin receptor interacting protein 5; ARIP2; FLJ11271; Mitochondrial outer membrane protein 25; OMP25FLJ41973; synaptojanin 2 binding protein; synaptojanin-2-binding protein Synj2bp|A, AA409442, ARIP2, ActRIP4, D12Wsu118, D12Wsu118e, OMP, OMP25|synaptojanin 2 binding protein|synaptojanin-2-binding protein|activin receptor interacting protein 2|activin receptor-interacting protein 4|mitochondrial outer membrane protein 25|outer membrane protein 25
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYNJ2BP, check out the SYNJ2BP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYNJ2BP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SYNJ2BP Antibody Picoband® (A11249-1)
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