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Product Info Summary
| SKU: | A04862-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SYNGAP1 Antibody Picoband®
SKU/Catalog Number
A04862-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SYNGAP1 Antibody Picoband® catalog # A04862-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SYNGAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04862-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human SYNGAP1 recombinant protein (Position: R3-R1175). Human SYNGAP1 shares 99% and 99.1% amino acid (aa) sequence identity with mouse and rat SYNGAP1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04862-1 is reactive to SYNGAP1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
148 kDa
Calculated molecular weight
148.284kDa
Background of SynGAP
Synaptic Ras GTPase-activating protein 1, also known as synaptic Ras-GAP 1 or SYNGAP1, is a protein that in humans is encoded by the SYNGAP1 gene. This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Allelic variants of this gene are associated with intellectual disability and autism spectrum disorder. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04862-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SYNGAP1 using anti-SYNGAP1 antibody (A04862-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNGAP1 antigen affinity purified polyclonal antibody (Catalog # A04862-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYNGAP1 at approximately 148 kDa. The expected band size for SYNGAP1 is at 148 kDa.
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Figure 2. Flow Cytometry analysis of K562 cells using anti-SYNGAP1 antibody (A04862-1).
Overlay histogram showing K562 cells stained with A04862-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYNGAP1 Antibody (A04862-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For SYNGAP1 (Source: Uniprot.org, NCBI)
Gene Name
SYNGAP1
Full Name
Ras/Rap GTPase-activating protein SynGAP
Weight
148.284kDa
Alternative Names
DKFZp761G1421; KIAA1938135kDa; MRD5; synaptic Ras GTPase activating protein 1 homolog (rat); synaptic Ras GTPase activating protein 1; Synaptic Ras-GAP 1 SYNGAP1 MRD5, RASA1, RASA5, SYNGAP synaptic Ras GTPase activating protein 1 ras/Rap GTPase-activating protein SynGAP|Ras GTPase-activating protein SynGAP|neuronal RasGAP|synaptic Ras GTPase activating protein 1 homolog|synaptic Ras GTPase activating protein, 135kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYNGAP1, check out the SYNGAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYNGAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SYNGAP1 Antibody Picoband® (A04862-1)
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