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Product Info Summary
| SKU: | A11201 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-synaptophysin 2 Synpr Antibody
View all Synaptoporin Antibodies
SKU/Catalog Number
A11201
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-synaptophysin 2 Synpr Antibody (Catalog # A11201). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-synaptophysin 2 Synpr Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11201)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.257~261(G-P-T-S-F)derived from Rat synaptophysin 2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11201 is reactive to SYNPR in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
36748 MW
Background of Synaptoporin
Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).
Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
Valtorta, F. et al. (2004) Bioessays 26, 445-53.
Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
Bonanomi, D. et al. (2007) Biochem J 404, 525-34.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11201 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 37kd
Western blotting: 1:500~1:1000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Synpr using anti-Synpr antibody (A11201).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synpr antigen affinity purified polyclonal antibody (Catalog # A11201) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Synpr.
Protein Target Info & Infographic
Gene/Protein Information For SYNPR (Source: Uniprot.org, NCBI)
Gene Name
SYNPR
Full Name
Synaptoporin
Weight
36748 MW
Superfamily
synaptophysin/synaptobrevin family
Alternative Names
Ras association domain-containing protein 4;RASSF4;AD037; Synpr|synaptoporin|synaptoporin|synaptorin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYNPR, check out the SYNPR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYNPR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-synaptophysin 2 Synpr Antibody (A11201)
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No publications found for A11201
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-synaptophysin 2 Synpr Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for caudate nucleus using anti-synaptophysin 2 antibody A11201. Let me know if you need anything else.
N. Zhang
Verified customer
Asked: 2019-09-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-20
Question
We are currently using anti-synaptophysin 2 antibody A11201 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
The anti-synaptophysin 2 antibody (A11201) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-20
Question
Can you help my question with product A11201, anti-synaptophysin 2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11201 anti-synaptophysin 2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-07
Question
Is this A11201 anti-synaptophysin 2 antibody reactive to the isotypes of SYNPR?
L. Baker
Verified customer
Asked: 2016-09-13
Answer
The immunogen of A11201 anti-synaptophysin 2 antibody is Peptide sequence around aa.257~261 (G-P-T-S-F) derived from Rat synaptophysin 2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-09-13
Question
Does anti-synaptophysin 2 antibody A11201 work on primate WB with brain retina?
G. Wu
Verified customer
Asked: 2013-07-16
Answer
Our lab technicians have not validated anti-synaptophysin 2 antibody A11201 on primate. You can run a BLAST between primate and the immunogen sequence of anti-synaptophysin 2 antibody A11201 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain retina in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-16
Question
Here is the WB image, lot number and protocol we used for caudate nucleus using anti-synaptophysin 2 antibody A11201. Please let me know if you require anything else.
B. Jones
Verified customer
Asked: 2013-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-05-30
