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Product Info Summary
| SKU: | M01236-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-STUB1 Rabbit Monoclonal Antibody
View all CHIP/STUB1 Antibodies
SKU/Catalog Number
M01236-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-STUB1 Rabbit Monoclonal Antibody catalog # M01236-2. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STUB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01236-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
19S73
Isotype
IgG
Immunogen
A synthesized peptide derived from human STUB1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01236-2 is reactive to STUB1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
35 kDa
Calculated molecular weight
38224 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01236-2 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IP 1:80
FC 1:50
Positive Control
WB: human 293T whole cell, human MCF-7 whole cell, human Hela whole cell, human THP-1 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STUB1 using anti-STUB1 antibody (M01236-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STUB1 antigen affinity purified monoclonal antibody (Catalog # M01236-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STUB1 at approximately 35 kDa. The expected band size for STUB1 is at 35 kDa.
Protein Target Info & Infographic
Gene/Protein Information For STUB1 (Source: Uniprot.org, NCBI)
Gene Name
STUB1
Full Name
E3 ubiquitin-protein ligase CHIP
Weight
38224 MW
Alternative Names
HLA class I histocompatibility antigen, alpha chain G;HLA G antigen;MHC class I antigen G;HLA-G;HLA-6.0, HLAG; STUB1 CHIP, HSPABP2, NY-CO-7, SCA48, SCAR16, SDCCAG7, UBOX1 STIP1 homology and U-box containing protein 1 E3 ubiquitin-protein ligase CHIP|CLL-associated KW-8|RING-type E3 ubiquitin transferase CHIP|STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase| NY-CO-7|carboxy terminus of Hsp70-interacting protein|heat shock protein A binding protein 2 (c-terminal)|serologically defined colon cancer 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STUB1, check out the STUB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STUB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STUB1 Rabbit Monoclonal Antibody (M01236-2)
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