Anti-STRADA Antibody (C-term)

Product Name

Anti-STRADA Antibody (C-term)

View all LYK5 Antibodies

SKU/Catalog Number

A01112-2

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-STRADA Antibody (C-term) (Catalog # A01112-2). Tested in WB, IHC-P, IF, Flow Cytometry application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-STRADA Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01112-2)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This STRADA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 303-346 amino acids from the C-terminal region of human STRADA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01112-2 is reactive to Strada in Human, Mouse

Applications

A01112-2 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

48369 Da

Background of LYK5

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IF: 1:25
IHC-P: 1:100
FC: 1:25

Validation Images & Assay Conditions

Click image to see more details

All lanes : Anti-STRADA Antibody (C-term) at 1:1000 dilution
Lane 1: 293 whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: Molt-4 whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 48 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

Fluorescent image of A549 cells stained with STRADA Antibody (C-term). A01112-2 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Click image to see more details

All lanes : Anti-STRADA Antibody (C-term) at 1:1000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Molt-4 cell lysate
Lane 3: Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 48 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

Immunohistochemical analysis of A01112-2 on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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Flow cytometric analysis of SH-SY5Y cells using STRADA Antibody (C-term)(green, Cat#A01112-2) compared to an isotype control of rabbit IgG(blue). A01112-2 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Gene/Protein Information For Strada (Source: Uniprot.org, NCBI)

Alternative Names

LYK5FLJ90524; NY-BR-96; protein kinase LYK5; Serologically defined breast cancer antigen NY-BR-96; STE20-like pseudokinase; STE20-related adapter protein; STE20-related adaptor protein; STE20-related kinase adapter protein alpha; STE20-related kinase adaptor alpha; Stlk; STRAD alpha; STRADPMSE Strada|2610019A05Rik, 6030402H20Rik, AI480680, E130112C09Rik|STE20-related kinase adaptor alpha|STE20-related kinase adapter protein alpha|STE20-related adapter protein|STRAD alpha|protein kinase LYK5

For more info on Strada, check out the Strada Infographic

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In this infographic, you will see the following information for Strada: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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