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Product Info Summary
| SKU: | A16012-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-STOX2 Antibody Picoband®
View all Storkhead Box 2 Antibodies
SKU/Catalog Number
A16012-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-STOX2 Antibody Picoband® catalog # A16012-1. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-STOX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16012-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human STOX2 recombinant protein (Position: D57-K659). Human STOX2 shares 95.3% amino acid (aa) sequence identity with mouse STOX2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A16012-1 is reactive to STOX2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
102 kDa
Calculated molecular weight
102.671kDa
Background of Storkhead Box 2
storkhead box 2
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16012-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human SH-SY5Y whole cell lysates, human Hela whole cell lysates, human 293T whole cell lysates, human RT4 whole cell lysates
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STOX2 using anti-STOX2 antibody (A16012-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STOX2 antigen affinity purified polyclonal antibody (Catalog # A16012-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STOX2 at approximately 102 kDa. The expected band size for STOX2 is at 102 kDa.
Protein Target Info & Infographic
Gene/Protein Information For STOX2 (Source: Uniprot.org, NCBI)
Gene Name
STOX2
Full Name
Storkhead-box protein 2
Weight
102.671kDa
Alternative Names
KIAA1392; Storkhead-Box Protein 2; STOX2; STOX-2 STOX2 storkhead box 2 storkhead-box protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STOX2, check out the STOX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STOX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STOX2 Antibody Picoband® (A16012-1)
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