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Product Info Summary
| SKU: | A05894-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ST8SIA2 Antibody Picoband®
View all ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibodies
SKU/Catalog Number
A05894-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-ST8SIA2 Antibody Picoband® catalog # A05894-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ST8SIA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05894-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ST8SIA2 recombinant protein (Position: D69-R267).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05894-1 is reactive to ST8SIA2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
42.43kDa
Background of ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2
Alpha-2,8-sialyltransferase 8B is an enzyme that in humans is encoded by the ST8SIA2 gene. The protein encoded by this gene is a type II membrane protein that is thought to catalyze the transfer of sialic acid from CMP-sialic acid to N-linked oligosaccharides and glycoproteins. The encoded protein may be found in the Golgi apparatus and may be involved in the production of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). This protein is a member of glycosyltransferase family 29.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05894-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat kidney tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ST8SIA2 using anti-ST8SIA2 antibody (A05894-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST8SIA2 antigen affinity purified polyclonal antibody (Catalog # A05894-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST8SIA2 at approximately 39 kDa. The expected band size for ST8SIA2 is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ST8SIA2 (Source: Uniprot.org, NCBI)
Gene Name
ST8SIA2
Full Name
Alpha-2,8-sialyltransferase 8B
Weight
42.43kDa
Superfamily
glycosyltransferase 29 family
Alternative Names
Troponin T, fast skeletal muscle; TnTf; Beta-TnTF; Fast skeletal muscle troponin T; fTnT; TNNT3 ST8SIA2 HsT19690, SIAT8-B, SIAT8B, ST8SIA-II, ST8SiaII, STX ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 alpha-2,8-sialyltransferase 8B|ST8 alpha-N-acetylneuraminate alpha-2,8-sialyltransferase 2|alpha-2,8-sialyltransferase 8B 1|sialyltransferase 8 (alpha-2, 8-sialytransferase) B|sialyltransferase 8B|sialyltransferase St8Sia II|sialyltransferase X
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ST8SIA2, check out the ST8SIA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ST8SIA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ST8SIA2 Antibody Picoband® (A05894-1)
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