Anti-ST5 Antibody Picoband®

Product Name

Anti-ST5 Antibody Picoband®

View all DENND2B Antibodies

SKU/Catalog Number

PA2287

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ST5 Antibody catalog # PA2287. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ST5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2287)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ST5, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2287 is reactive to ST5 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

126 kDa

Calculated molecular weight

126485 MW

Background of DENND2B

Suppression of tumorigenicity 5 is a protein that in humans is encoded by the ST5 gene. This gene is mapped to 11p15.4. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA2287 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: Rat Testis Tissue, A431 Whole Cell, HELA Whole Cell, COLO320 Whole Cell, NIH/3T3 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For ST5 (Source: Uniprot.org, NCBI)

Alternative Names

Suppression of tumorigenicity 5 protein;DENN domain-containing protein 2B;HeLa tumor suppression 1;ST5;DENND2B, HTS1; DENND2B HTS1, ST5, p126 DENN domain containing 2B DENN domain-containing protein 2B|DENN/MADD domain containing 2B|heLa tumor suppression 1|suppression of tumorigenicity 5 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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