Product Info Summary
SKU: | A11609-3 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SSH3 Antibody Picoband™
SKU/Catalog Number
A11609-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SSH3 Antibody Picoband™ catalog # A09748-1. Tested in WB, IHC, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SSH3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11609-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human SSH3 recombinant protein (Position: Q108-Q620). Human SSH3 shares 82.8% and 84.2% amino acid (aa) sequence identity with mouse and rat SSH3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A11609-3 is reactive to SSH3 in Human, Rat
Applications
A11609-3 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
95 kDa
Calculated molecular weight
52588 MW
Background of SSH3
Protein phosphatase Slingshot homolog 3 is an enzyme that in humans is encoded by the SSH3 gene. The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1. The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human, Rat
Immunohistochemistry, 2-5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a11609 3 ssh3 primary antibodies wb testing 1 a11609 3 ssh3 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a11609-3-ssh3-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of SSH3 using anti-SSH3 antibody (A11609-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293Twhole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3 antigen affinity purified polyclonal antibody (Catalog # A11609-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSH3 at approximately 95 kDa. The expected band size for SSH3 is at 73 kDa.
![a11609 3 ssh3 primary antibodies ihc testing 2 a11609 3 ssh3 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a11609-3-ssh3-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of SSH3 using anti-SSH3 antibody (A11609-3).
SSH3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSH3 Antibody (A11609-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a11609 3 ssh3 primary antibodies ihc testing 3 a11609 3 ssh3 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a11609-3-ssh3-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of SSH3 using anti-SSH3 antibody (A11609-3).
SSH3 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSH3 Antibody (A11609-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a11609 3 ssh3 primary antibodies ihc testing 4 a11609 3 ssh3 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/1/a11609-3-ssh3-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of SSH3 using anti-SSH3 antibody (A11609-3).
SSH3 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSH3 Antibody (A11609-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a11609 3 ssh3 primary antibodies if testing 5 a11609 3 ssh3 primary antibodies if testing 5](https://www.bosterbio.com/media/catalog/product/a/1/a11609-3-ssh3-primary-antibodies-if-testing-5.jpg)
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Figure 5. IF analysis of SSH3 using anti-SSH3 antibody (A11609-3) and anti-Beta Tubulin antibody (M01857-3).
SSH3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SSH3 Antibody (A11609-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a11609 3 ssh3 primary antibodies fcm testing 6 a11609 3 ssh3 primary antibodies fcm testing 6](https://www.bosterbio.com/media/catalog/product/a/1/a11609-3-ssh3-primary-antibodies-fcm-testing-6.png)
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Figure 6. Flow Cytometry analysis of RT4 cells using anti-SSH3 antibody (A11609-3).
Overlay histogram showing RT4 cells stained with A11609-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSH3 Antibody (A11609-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For SSH3 (Source: Uniprot.org, NCBI)
Gene Name
SSH3
Full Name
Protein phosphatase Slingshot homolog 3
Weight
52588 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
EC 3.1.3.16; EC 3.1.3.48; FLJ10928; FLJ20515; FLJ42240; hSSH-3L; protein phosphatase Slingshot homolog 3; slingshot 3; slingshot homolog 3 (Drosophila); SSH3; SSH3L; SSH-3L; SSH-like protein 3 SSH3 SSH3L slingshot protein phosphatase 3 protein phosphatase Slingshot homolog 3|SSH-3L|SSH-like protein 3|hSSH-3L|slingshot 3|slingshot homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SSH3, check out the SSH3 Infographic
![SSH3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SSH3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SSH3 Antibody Picoband™ (A11609-3)
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