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Product Info Summary
| SKU: | A07709-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SRP14 Antibody Picoband®
SKU/Catalog Number
A07709-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SRP14 Antibody Picoband® catalog # A07709-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SRP14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07709-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SRP14 recombinant protein (Position: M1-D92).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07709-1 is reactive to SRP14 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
49229 MW
Background of SRP14
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07709-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SRP14 using anti-SRP14 antibody (A07709-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRP14 antigen affinity purified polyclonal antibody (Catalog # A07709-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRP14 at approximately 18 kDa. The expected band size for SRP14 is at 15 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-SRP14 antibody (A07709-1).
Overlay histogram showing 293T cells stained with A07709-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRP14 Antibody (A07709-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SRP14 (Source: Uniprot.org, NCBI)
Gene Name
SRP14
Full Name
Signal recognition particle 14 kDa protein
Weight
49229 MW
Superfamily
SRP14 family
Alternative Names
Protein Hook homolog 3; h-hook3; Hhk3; HOOK3 SRP14 ALURBP signal recognition particle 14 signal recognition particle 14 kDa protein|18 kDa Alu RNA-binding protein|homologous Alu RNA binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRP14, check out the SRP14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRP14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SRP14 Antibody Picoband® (A07709-1)
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