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Product Info Summary
| SKU: | M00704 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-SRD5A2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00704
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-SRD5A2 Rabbit Monoclonal Antibody catalog # M00704. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SRD5A2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00704)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
24S47
Isotype
IgG
Immunogen
A synthesized peptide derived from human SRD5A2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00704 is reactive to SRD5A2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated molecular weight
48551 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00704 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: human PC-3 whole cell, human RT4 whole cell, human SH-SY5Y whole cell, human HepG2 whole cell, human NIH/3T3 whole cell, mouse HT22 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SRD5A2 using anti-SRD5A2 antibody (M00704).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human NIH/3T3 whole cell lysates,
Lane 6: mouse HT22 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRD5A2 antigen affinity purified monoclonal antibody (Catalog # M00704) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRD5A2 at approximately 28 kDa. The expected band size for SRD5A2 is at 28 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SRD5A2 (Source: Uniprot.org, NCBI)
Gene Name
SRD5A2
Full Name
3-oxo-5-alpha-steroid 4-dehydrogenase 2
Weight
48551 MW
Superfamily
steroid 5-alpha reductase family
Alternative Names
Cyclin-A2;Cyclin-A;CCNA2;CCN1, CCNA; SRD5A2 steroid 5 alpha-reductase 2 3-oxo-5-alpha-steroid 4-dehydrogenase 2|5 alpha-SR2|S5AR 2|SR type 2|steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)|type II 5-alpha reductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRD5A2, check out the SRD5A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRD5A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SRD5A2 Rabbit Monoclonal Antibody (M00704)
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