Product Info Summary
SKU: | M00107-2 |
---|---|
Size: | 100µg |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-Src Monoclonal Antibody (5A18)
SKU/Catalog Number
M00107-2
Size
100µg
Form
Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Description
Mouse Monoclonal 5A18 antibody for SRC detection. Tested positive for IHC, WB in Human, Mouse, Rat
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Src Monoclonal Antibody (5A18) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00107-2)
Host
Mouse
Contents
Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
5A18
Isotype
IgG2b, kappa
Immunogen
Recombinant human full length c-Src.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00107-2 is reactive to SRC in Human, Mouse, Rat
Applications
M00107-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
34310 MW
Background of SRC
SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SRC using anti-SRC antibody (M00107-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRC antigen affinity purified polyclonal antibody (Catalog # M00107-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for SRC.
Click image to see more details
Figure 2. IHC analysis of SRC using anti-SRC antibody (M00107-2).
SRC was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SRC Antibody (M00107-2) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SRC using anti-SRC antibody (M00107-2).
SRC was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SRC Antibody (M00107-2) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SRC (Source: Uniprot.org, NCBI)
Gene Name
SRC
Full Name
Proto-oncogene tyrosine-protein kinase Src
Weight
34310 MW
Superfamily
protein kinase superfamily
Alternative Names
ASV; c-Src; EC 2.7.10; EC 2.7.10.2; pp60c-src; Rous sarcoma; RSVgp4; Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) SRC ASV1, THC6, c-SRC, p60-Src, SRC SRC proto-oncogene, non-receptor tyrosine kinase proto-oncogene tyrosine-protein kinase Src|proto-oncogene c-Src|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1|v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRC, check out the SRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Src Monoclonal Antibody (5A18) (M00107-2)
Hello CJ!
M00107-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Qq, Zhou Dl, Lei Y, Zheng L, Chen Sx, Gou Hj, Gu Ql, He Xd, Lan T, Qi Cl, Li Jc, Ding Yq, Qiao L, Wang Lj. Oncotarget. 2015 Feb 20;6(5):3123-35. Slit2/Robo1 Signaling Promotes Intestinal Tumorigenesis Through Src-Mediated Activation Of The W...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Src Monoclonal Antibody (5A18)
Question
We have observed staining in mouse platelet. Do you have any suggestions? Is anti-Src Monoclonal antibody (5A18) supposed to stain platelet positively?
K. Mitchell
Verified customer
Asked: 2020-02-21
Answer
From what I have seen in literature platelet does express SRC. From what I have seen in Uniprot.org, SRC is expressed in fundus of stomach, lung skin, platelet, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, among other tissues. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2020-02-21
Question
I see that the anti-Src Monoclonal antibody (5A18) M00107-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-11
Question
Please see the WB image, lot number and protocol we used for fundus of stomach using anti-Src Monoclonal antibody (5A18) M00107-2. Please let me know if you require anything else.
R. Dhar
Verified customer
Asked: 2019-09-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
I am looking for to test anti-Src Monoclonal antibody (5A18) M00107-2 on rat fundus of stomach for research purposes, then I may be interested in using anti-Src Monoclonal antibody (5A18) M00107-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The products we sell, including anti-Src Monoclonal antibody (5A18) M00107-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-18
Question
We purchased anti-Src Monoclonal antibody (5A18) for WB on leukemic t-cell in the past. I am using mouse, and We intend to use the antibody for IHC next. I would like examining leukemic t-cell as well as lung skin in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
I looked at the website and datasheets of our anti-Src Monoclonal antibody (5A18) and it seems that M00107-2 has been tested on mouse in both WB and IHC. Thus M00107-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-16
Question
Is there a BSA free version of anti-Src Monoclonal antibody (5A18) M00107-2 available?
N. Jackson
Verified customer
Asked: 2018-07-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Src Monoclonal antibody (5A18) M00107-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-26
Question
We are currently using anti-Src Monoclonal antibody (5A18) M00107-2 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The anti-Src Monoclonal antibody (5A18) (M00107-2) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-12
Question
Is a blocking peptide available for product anti-Src Monoclonal antibody (5A18) (M00107-2)?
T. Mitchell
Verified customer
Asked: 2017-02-23
Answer
We do provide the blocking peptide for product anti-Src Monoclonal antibody (5A18) (M00107-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-02-23
Question
I was wanting to use your anti-Src Monoclonal antibody (5A18) for IHC for rat fundus of stomach on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fundus of stomach identification?
H. Taylor
Verified customer
Asked: 2017-02-09
Answer
As indicated on the product datasheet, M00107-2 anti-Src Monoclonal antibody (5A18) has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fundus of stomach in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fundus of stomach using anti-Src Monoclonal antibody (5A18) M00107-2. Let me know if you need anything else.
D. Mangal
Verified customer
Asked: 2017-01-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-09
Question
Does anti-Src Monoclonal antibody (5A18) M00107-2 work for IHC with fundus of stomach?
N. Edwards
Verified customer
Asked: 2016-10-10
Answer
According to the expression profile of fundus of stomach, SRC is highly expressed in fundus of stomach. So, it is likely that anti-Src Monoclonal antibody (5A18) M00107-2 will work for IHC with fundus of stomach.
Boster Scientific Support
Answered: 2016-10-10
Question
Our lab were happy with the WB result of your anti-Src Monoclonal antibody (5A18) . However we have observed positive staining in erythroleukemia cell membrane. using this antibody. Is that expected? Could you tell me where is SRC supposed to be expressed?
M. Carter
Verified customer
Asked: 2016-04-01
Answer
From literature, erythroleukemia does express SRC. Generally SRC expresses in cell membrane. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2016-04-01
Question
Is this M00107-2 anti-Src Monoclonal antibody (5A18) reactive to the isotypes of SRC?
N. Zhang
Verified customer
Asked: 2014-04-17
Answer
The immunogen of M00107-2 anti-Src Monoclonal antibody (5A18) is Recombinant human full length c-Src. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-04-17
Question
Would M00107-2 anti-Src Monoclonal antibody (5A18) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Taylor
Verified customer
Asked: 2013-08-02
Answer
You can see on the product datasheet, M00107-2 anti-Src Monoclonal antibody (5A18) as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-02
Question
I have a question about product M00107-2, anti-Src Monoclonal antibody (5A18) . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Parker
Verified customer
Asked: 2013-03-25
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00107-2 anti-Src Monoclonal antibody (5A18) , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-03-25