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Product Info Summary
| SKU: | A12999 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-SPTBN5 Antibody
SKU/Catalog Number
A12999
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SPTBN5 Antibody (Catalog # A12999). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPTBN5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12999)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SPTBN5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12999 is reactive to SPTBN5 in Human
Applications
A12999 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
416750 MW
Background of SPTBN5
Belongs to the spectrin family.
Stabach P.R., J. Biol. Chem. 275:21385-21395(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using SPTBN5 antibody A12999.
Protein Target Info & Infographic
Gene/Protein Information For SPTBN5 (Source: Uniprot.org, NCBI)
Gene Name
SPTBN5
Full Name
Spectrin beta chain, non-erythrocytic 5
Weight
416750 MW
Superfamily
spectrin family
Alternative Names
Spectrin beta chain, non-erythrocytic 5 SPTBN5 BSPECV, HUBSPECV, HUSPECV spectrin beta, non-erythrocytic 5 spectrin beta chain, non-erythrocytic 5|beta V spectrin|spectrin beta chain, brain 4|spectrin, non-erythroid beta chain 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPTBN5, check out the SPTBN5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPTBN5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPTBN5 Antibody (A12999)
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No publications found for A12999
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SPTBN5 Antibody
Question
I have a question about product A12999, anti-SPTBN5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12999 anti-SPTBN5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-19
Question
Is there a BSA free version of anti-SPTBN5 antibody A12999 available?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SPTBN5 antibody A12999 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-20
Question
Our lab want to know about to test anti-SPTBN5 antibody A12999 on human cerebellum for research purposes, then I may be interested in using anti-SPTBN5 antibody A12999 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The products we sell, including anti-SPTBN5 antibody A12999, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-12
Question
Does anti-SPTBN5 antibody A12999 work for IHC with cerebellum?
Verified Customer
Verified customer
Asked: 2019-03-25
Answer
According to the expression profile of cerebellum, SPTBN5 is highly expressed in cerebellum. So, it is likely that anti-SPTBN5 antibody A12999 will work for IHC with cerebellum.
Boster Scientific Support
Answered: 2019-03-25
Question
Is this A12999 anti-SPTBN5 antibody reactive to the isotypes of SPTBN5?
R. Jones
Verified customer
Asked: 2019-02-28
Answer
The immunogen of A12999 anti-SPTBN5 antibody is Synthesized peptide derived from internal of human SPTBN5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-28
Question
Is a blocking peptide available for product anti-SPTBN5 antibody (A12999)?
B. Jones
Verified customer
Asked: 2017-12-26
Answer
We do provide the blocking peptide for product anti-SPTBN5 antibody (A12999). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-26
Question
We are currently using anti-SPTBN5 antibody A12999 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
G. Miller
Verified customer
Asked: 2014-01-16
Answer
The anti-SPTBN5 antibody (A12999) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-01-16
