Product Info Summary
SKU: | M03831-1 |
---|---|
Size: | 80 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SPTAN1 Antibody (Center)
View all Alpha Fodrin Antibodies
SKU/Catalog Number
M03831-1
Size
80 µl
Description
Boster Bio Anti-SPTAN1 Antibody (Center) (Catalog # M03831-1). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SPTAN1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03831-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This SPTAN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 977-991 amino acids from the Central region of human SPTAN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03831-1 is reactive to SPTAN1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
Calculated molecular weight
284539 Da
Background of Alpha Fodrin
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03831-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Positive Control
Validation Images & Assay Conditions
![m03831 1 anti sptan1 antibody center wb testing 1 m03831 1 anti sptan1 antibody center wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m03831-1-anti-sptan1-antibody-center-wb-testing-1.jpg)
Click image to see more details
All lanes : Anti-SPTAN1 Antibody (Center) at 1:1000 dilution
Lane 1: Hela whole cell lysates
Lane 2: NIH/3T3 whole cell lysates
Lane 3: rat kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 285 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
![m03831 1 anti sptan1 antibody center wb testing 2 m03831 1 anti sptan1 antibody center wb testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m03831-1-anti-sptan1-antibody-center-wb-testing-2.jpg)
Click image to see more details
Anti-SPTAN1 Antibody (Center) at 1:2000 dilution + human brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 285 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
![m03831 1 anti sptan1 antibody center wb testing 3 m03831 1 anti sptan1 antibody center wb testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m03831-1-anti-sptan1-antibody-center-wb-testing-3.jpg)
Click image to see more details
Anti-SPTAN1 Antibody (Center) at 1:2000 dilution + SH-SY5Y whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 285 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For SPTAN1 (Source: Uniprot.org, NCBI)
Gene Name
SPTAN1
Full Name
Spectrin alpha chain, non-erythrocytic 1
Weight
284539 Da
Superfamily
spectrin family
Alternative Names
Alpha-II spectrin; EIEE5; FLJ17738; FLJ44613; Fodrin alpha chain; NEAS; spectrin alpha chain, brain; spectrin, alpha, non-erythrocytic 1 (alpha-fodrin); Spectrin, non-erythroid alpha chain; SPTA2 SPTAN1 DEE5, EIEE5, NEAS, SPTA2 spectrin alpha, non-erythrocytic 1 spectrin alpha chain, non-erythrocytic 1|alpha-II spectrin|alpha-fodrin|epididymis secretory sperm binding protein|fodrin alpha chain|spectrin, non-erythroid alpha chain|spectrin, non-erythroid alpha subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPTAN1, check out the SPTAN1 Infographic
![SPTAN1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPTAN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPTAN1 Antibody (Center) (M03831-1)
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