Product Info Summary
SKU: | A15117 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SPRYD5 TRIM51 Antibody
SKU/Catalog Number
A15117
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SPRYD5 TRIM51 Antibody (Catalog # A15117). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SPRYD5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SPRYD5 TRIM51 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15117)
Host
Rabbit
Contents
SPRYD5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SPRYD5 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD5. The immunogen is located within amino acids 350 - 400 of SPRYD5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SPRYD5 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD5 are known to exist; this antibody will detect both isoforms
Reactive Species
A15117 is reactive to TRIM51 in Human, Mouse, Rat
Applications
A15117 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
52285 MW
Background of SPRYD5
The SPRY domain-containing protein 5 (SPRYD5) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD5 belongs to the TRIM/RBCC family and contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger. Little is known of the function of the SPRYD5 protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SPRYD5 antibody can be used for detection of SPRYD5 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
![a15117 anti spryd5 trim51 antibody wb testing 1 a15117 anti spryd5 trim51 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a15117-anti-spryd5-trim51-antibody-wb-testing-1.jpg)
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Western blot analysis of SPRYD5 in rat brain tissue lysate with SPRYD5 antibody at (A) 0.25 and (B) 0.5 μg/mL .
![a15117 anti spryd5 trim51 antibody ihc testing 2 a15117 anti spryd5 trim51 antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a15117-anti-spryd5-trim51-antibody-ihc-testing-2.jpg)
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Immunohistochemistry of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 5 μg/mL.
![a15117 anti spryd5 trim51 antibody if testing 3 a15117 anti spryd5 trim51 antibody if testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a15117-anti-spryd5-trim51-antibody-if-testing-3.jpg)
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Immunofluorescence of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TRIM51 (Source: Uniprot.org, NCBI)
Gene Name
TRIM51
Full Name
Tripartite motif-containing protein 51
Weight
52285 MW
Superfamily
TRIM/RBCC family
Alternative Names
MGC10977; SPRY domain containing 5; SPRY domain-containing protein 5; TRIM51; tripartite motif-containing 51; Tripartite motif-containing protein 51 TRIM51 SPRYD5A, TRIM51 tripartite motif-containing 51 tripartite motif-containing protein 51|SPRY domain containing 5|SPRY domain-containing protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM51, check out the TRIM51 Infographic
![TRIM51 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM51: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPRYD5 TRIM51 Antibody (A15117)
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