Anti-Spred2 Antibody

Product Name

Anti-Spred2 Antibody

View all Spred2 Antibodies

SKU/Catalog Number

A06368

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Spred2 Antibody (Catalog # A06368). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Spred2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Spred2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06368)

Host

Rabbit

Contents

Spred2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Spred2 antibody was raised against a 13 amino acid synthetic peptide near the center of the human Spred2. The immunogen is located within amino acids 140 - 190 of Spred2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.

Reactive Species

A06368 is reactive to SPRED2 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

47558 MW

Background of Spred2

Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. At least two isoforms of Spred2 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06368 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For SPRED2 (Source: Uniprot.org, NCBI)

Alternative Names

Spred-2, Sprouty-related, EVH1 domain-containing protein 2, Spred-2 Spred2|C79158, Spre|sprouty-related EVH1 domain containing 2|sprouty-related, EVH1 domain-containing protein 2|sprouty protein with EVH-1 domain 2, related sequence

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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