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Product Info Summary
| SKU: | A03350 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-AFAP1L2 Antibody
SKU/Catalog Number
A03350
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AFAP1L2 Antibody (Catalog # A03350). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AFAP1L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-AFAP1L2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03350)
Host
Rabbit
Contents
AFAP1L2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AFAP1L2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AFAP1L2. The immunogen is located within the last 50 amino acids of AFAP1L2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A.
Reactive Species
A03350 is reactive to AFAP1L2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
91300 MW
Background of AFAP1L2
AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03350 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AFAP1L2 antibody can be used for detection of AFAP1L2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AFAP1L2 in mouse liver tissue lysate with AFAP1L2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of AFAP1L2 in human liver tissue with AFAP1L2 antibody at 2.5 μg/mL.
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Immunofluorescence of AFAP1L2 in human liver tissue with AFAP1L antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For AFAP1L2 (Source: Uniprot.org, NCBI)
Gene Name
AFAP1L2
Full Name
Actin filament-associated protein 1-like 2
Weight
91300 MW
Alternative Names
XB130, KIAA1914, CTB-1144G6.4, XB130, Actin filament-associated protein 1-like 2, AFAP1-like protein 2 AFAP1L2 CTB-1144G6.4, KIAA1914, XB130 actin filament associated protein 1 like 2 actin filament-associated protein 1-like 2|AFAP1-like protein 2|CTB-1144G6.6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AFAP1L2, check out the AFAP1L2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AFAP1L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AFAP1L2 Antibody (A03350)
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