Product Info Summary
SKU: | M01390 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-SPHK1 Monoclonal Antibody
View all Sphingosine Kinase 1/SPHK1 Antibodies
SKU/Catalog Number
M01390
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SPHK1 Monoclonal Antibody catalog # M01390. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPHK1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01390)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACCB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SPHK1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01390 is reactive to SPHK1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120-140 kDa
Calculated molecular weight
62094 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01390 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SPHK1 expression in Raji cell lysate.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For SPHK1 (Source: Uniprot.org, NCBI)
Gene Name
SPHK1
Full Name
Sphingosine kinase 1
Weight
62094 MW
Alternative Names
Interferon-induced, double-stranded RNA-activated protein kinase;2.7.11.1;Eukaryotic translation initiation factor 2-alpha kinase 2;eIF-2A protein kinase 2;Interferon-inducible RNA-dependent protein kinase;P1/eIF-2A protein kinase;Protein kinase RNA-activated;PKR;Protein kinase R ;Tyrosine-protein kinase EIF2AK2;2.7.10.2;p68 kinase;EIF2AK2;PKR, PRKR; SPHK1 SPHK sphingosine kinase 1 sphingosine kinase 1|SK 1|SPK 1|acetyltransferase SPHK1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPHK1, check out the SPHK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPHK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPHK1 Monoclonal Antibody (M01390)
Hello CJ!
M01390 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen C, Gong W, Li C, Xiong F, Wang S, Huang J, Wang Y, Chen Z, Chen Q, Liu P, Lan T, Huang H. Oncotarget. 2017 Aug 10;8(45):78660-78676. doi: 10.18632/oncotarget.20205. eCollection 2017 Oct 3. Sphingosine kinase 1 mediates AGEs-induced fibronecti...
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Customer Q&As
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1 Customer Q&As for Anti-SPHK1 Monoclonal Antibody
Question
What is the sample target on Western blot analysis in your product image for M01390?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-SPHK1 Monoclonal Antibody (M01390) expression in Raji cell lysate.
Boster Scientific Support
Answered: 2019-12-10