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Product Info Summary
| SKU: | A14863 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SPATA6 Antibody
SKU/Catalog Number
A14863
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SPATA6 Antibody (Catalog # A14863). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SPATA6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SPATA6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14863)
Host
Rabbit
Contents
SPATA6 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SPATA6 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human SPATA6. The immunogen is located within amino acids 400 - 450 of SPATA6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of SPATA6 are known to exist; this antibody will detect only the longest two isoforms. SPATA6 antibody is predicted to not cross-react with other SPATA protein family members.
Reactive Species
A14863 is reactive to SPATA6 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
55989 MW
Background of SPATA6
The development of spermatogonial stem cells into spermatozoa, i.e., spermatogenesis, is a highly ordered process that requires multiple proteins, including some that are testis-specific or testis-specific isoforms of genes that are expressed in other tissues. One such protein is SPATA6: while its full-length isoform is expressed in multiple tissues including thymus, brain and testis, the shorter isoform is expressed only in testis. It has some homology to the motor domain of kinesin related proteins and with the C. elegans neural calcium sensor protein NCS-2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14863 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SPATA6 antibody can be used for detection of SPATA6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SPATA6 in A20 cell lysate with SPATA6 antibody at 1 μg/mL.
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Immunohistochemistry of SPATA6 in mouse testis tissue with SPATA6 antibody at 2.5 μg/mL.
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Immunofluorescence of SPATA6 in mouse testis tissue with SPATA6 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SPATA6 (Source: Uniprot.org, NCBI)
Gene Name
SPATA6
Full Name
Spermatogenesis-associated protein 6
Weight
55989 MW
Superfamily
SPATA6 family
Alternative Names
HASH, SRF1, SRF-1, Spermatogenesis-associated protein 6 SPATA6 HASH, SRF-1, SRF1 spermatogenesis associated 6 spermatogenesis-associated protein 6|spermatogenesis-related factor-1|testicular tissue protein Li 178
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPATA6, check out the SPATA6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPATA6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPATA6 Antibody (A14863)
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