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Product Info Summary
| SKU: | A16295 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SPATA3 Antibody
SKU/Catalog Number
A16295
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SPATA3 Antibody (Catalog # A16295). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SPATA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SPATA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16295)
Host
Rabbit
Contents
SPATA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SPATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPATA3. The immunogen is located within the last 50 amino acids of SPATA3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of SPATA3 are known to exist; this antibody will detect only the longest two isoforms. SPATA3 antibody is predicted to not cross-react with SPATA protein family members
Reactive Species
A16295 is reactive to SPATA3 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
20901 MW
Background of Spata3
The testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) protein, also known as SPATA3, is highly expressed in testis, and to a lower extent in other tissues. SPATA3 contains a highly conserved J domain, which is present in all DnaJ-like proteins, and is thus a novel member of the HSP40 protein family. SPATA3 is thought to be involved in apoptosis of spermatogenic cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16295 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SPATA3 in 3T3 cell lysate with SPATA3 antibody at 1 μg/mL.
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Immunohistochemistry of SPATA3 in mouse testis tissue with SPATA3 antibody at 2.5 μg/mL.
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Immunofluorescence of SPATA3 in mouse testis tissue with SPATA3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SPATA3 (Source: Uniprot.org, NCBI)
Gene Name
SPATA3
Full Name
Spermatogenesis-associated protein 3
Weight
20901 MW
Alternative Names
TSARG1, TSARG1, Spermatogenesis-associated protein 3, Testis and spermatogenesis cell-related protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPATA3, check out the SPATA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPATA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPATA3 Antibody (A16295)
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