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Product Info Summary
| SKU: | PB9559 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SPARC Antibody Picoband®
SKU/Catalog Number
PB9559
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SPARC Antibody Picoband® catalog # PB9559. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPARC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9559)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SPARC, different from the related mouse and rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9559 is reactive to SPARC in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
34632 MW
Background of SPARC
SPARC, secreted protein acidic and rich in cysteine , also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9559 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, 293T Whole Cell, MCF-7 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (PB9559).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HELA Whole Cell Lysate at 40ug,
Lane 2: 293T Whole Cell Lysate at 40ug,
Lane 3: MCF-7 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # PB9559) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 35 kDa. The expected band size for SPARC is at 35 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SPARC (Source: Uniprot.org, NCBI)
Gene Name
SPARC
Full Name
SPARC
Weight
34632 MW
Superfamily
SPARC family
Alternative Names
SPARC;Basement-membrane protein 40;BM-40;Osteonectin;ON;Secreted protein acidic and rich in cysteine;SPARC;ON; SPARC BM-40, OI17, ON, ONT secreted protein acidic and cysteine rich SPARC|basement-membrane protein 40|secreted protein, acidic, cysteine-rich (osteonectin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPARC, check out the SPARC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPARC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPARC Antibody Picoband® (PB9559)
Hello CJ!
PB9559 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
CD90 is upregulated in gastric cancer tissues and inhibits gastric cancer cell apoptosis by modulating the expression level of SPARC protein
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Customer Q&As
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4 Customer Q&As for Anti-SPARC Antibody Picoband®
Question
We have observed staining in human muscle. What should we do? Is anti-SPARC antibody supposed to stain muscle positively?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Based on literature muscle does express SPARC. Based on Uniprot.org, SPARC is expressed in tibia, placenta, muscle, pns skin, among other tissues. Regarding which tissues have SPARC expression, here are a few articles citing expression in various tissues:
Muscle, PNS, and Skin, Pubmed ID: 15489334
Placenta, Pubmed ID: 3410046
Boster Scientific Support
Answered: 2020-01-08
Question
We are currently using anti-SPARC antibody PB9559 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
K. Jackson
Verified customer
Asked: 2018-06-25
Answer
The anti-SPARC antibody (PB9559) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-25
Question
I am looking for using your anti-SPARC antibody for platelet degranulation studies. Has this antibody been tested with western blotting on 293t whole cell lysate? We would like to see some validation images before ordering.
C. Jones
Verified customer
Asked: 2016-09-20
Answer
We appreciate your inquiry. This PB9559 anti-SPARC antibody is validated on hela whole cell lysate, 293t whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-09-20
Question
My colleagues were happy with the WB result of your anti-SPARC antibody. However we have seen positive staining in muscle extracellular using this antibody. Is that expected? Could you tell me where is SPARC supposed to be expressed?
J. Krishna
Verified customer
Asked: 2014-09-11
Answer
Based on literature, muscle does express SPARC. Generally SPARC expresses in secreted, extracellular space, extracellular. Regarding which tissues have SPARC expression, here are a few articles citing expression in various tissues:
Muscle, PNS, and Skin, Pubmed ID: 15489334
Placenta, Pubmed ID: 3410046
Boster Scientific Support
Answered: 2014-09-11
