Anti-SPAG16 Antibody Picoband™

Product Name

Anti-SPAG16 Antibody Picoband™

View all Spag16 Antibodies

SKU/Catalog Number

A09080-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SPAG16 Antibody Picoband™ catalog # A12575-3. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SPAG16 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09080-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human SPAG16 recombinant protein (Position: D28-F619). Human SPAG16 shares 75.1% amino acid (aa) sequence identity with mouse SPAG16.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A09080-3 is reactive to Spag16 in Human, Mouse, Rat

Applications

A09080-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

71 kDa

Calculated molecular weight

52588 MW

Background of Spag16

Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SPAG16 using anti-SPAG16 antibody (A09080-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse Raw264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPAG16 antigen affinity purified polyclonal antibody (Catalog # A09080-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPAG16 at approximately 71 kDa. The expected band size for SPAG16 is at 71 kDa.

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Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-SPAG16 antibody (A09080-3).
Overlay histogram showing SH-SY5Y cells stained with A09080-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPAG16 Antibody (A09080-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Spag16 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp666P1710; FLJ22724; FLJ37717; Pf20 protein homolog; PF20MGC87036; sperm associated antigen 16; sperm-associated antigen 16 protein; sperm-associated WD repeat protein; WD repeat domain 29; WDR29 Spag16|4921511D23Rik, 4930524F24Rik, 4930585K05Rik, AV261009, Pf2, Pf20, Wdr2, Wdr29|sperm associated antigen 16|sperm-associated antigen 16 protein|pf20 protein homolog|sperm-associated WD repeat protein

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In this infographic, you will see the following information for Spag16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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