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Product Info Summary
| SKU: | A02077-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Sp7/Osterix Antibody Picoband®
View all Osterix/Sp7 Antibodies
SKU/Catalog Number
A02077-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Sp7/Osterix Antibody Picoband® catalog # A02077-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sp7/Osterix Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02077-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Sp7/Osterix recombinant protein (Position: A2-E241).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02077-1 is reactive to SP7 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
44.994kDa
Background of Osterix/Sp7
Transcription factor Sp7, also called Osterix (Osx), is a protein that in humans is encoded by the SP7 gene. This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02077-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HELA whole cell, human U87 whole cell, human MDA-MB453 whole cell, human MCF-7 whole cell, human Jurkat whole cell, human THP-1 whole cellrat lung tissue, rat thymus tissue, rat skeletal muscle tissue, rat testis tissue, mouse lung tissue, mouse thymus tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Sp7/Osterix using anti-Sp7/Osterix antibody (A02077-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human MDA-MB453 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human THP-1 whole cell lysates.
Lane 7: rat lung tissue lysates,
Lane 8: rat thymus tissue lysates,
Lane 9: rat skeletal muscle tissue lysates,
Lane 10: rat testis tissue lysates,
Lane 11: mouse lung tissue lysates,
Lane 12: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sp7/Osterix antigen affinity purified polyclonal antibody (Catalog # A02077-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sp7/Osterix at approximately 45KD. The expected band size for Sp7/Osterix is at 45KD.
Protein Target Info & Infographic
Gene/Protein Information For SP7 (Source: Uniprot.org, NCBI)
Gene Name
SP7
Full Name
Transcription factor Sp7
Weight
44.994kDa
Superfamily
Sp1 C2H2-type zinc-finger protein family
Alternative Names
Deleted in azoospermia-like; DAZ homolog; DAZ-like autosomal; Deleted in azoospermia-like 1; SPGY-like-autosomal; DAZL; DAZH; DAZL1; DAZLA; SPGYLA SP7 OI11, OI12, OSX, osterix Sp7 transcription factor transcription factor Sp7|zinc finger protein osterix
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SP7, check out the SP7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SP7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sp7/Osterix Antibody Picoband® (A02077-1)
Hello CJ!
A02077-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dynamic expression of Runx2, Osterix and AJ18 in the femoral head of steroid‐induced osteonecrosis in rats
Chen G,Wang Q,Li Z,Yang Q,Liu Y,Du Z,Zhang G,Song Y.Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head.Bone.2020 Apr;133:115258.doi:10.1016/j.bone.2020.115258.Epu
Species: Human
A02077-1 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA
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