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Product Info Summary
| SKU: | A05013-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SOX7 Antibody Picoband®
SKU/Catalog Number
A05013-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SOX7 Antibody Picoband® catalog # A05013-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05013-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SOX7 recombinant protein (Position: K57-D343).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05013-1 is reactive to SOX7 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
25847 MW
Background of Sox7
SOX7 belongs to SOX gene family and SOX proteins are transcription factors with critical roles in the regulation of diverse developmental processes. This gene is mapped to 8p23.1. SOX7 gene contains at least 2 exons. In cotransfected 293 cells, Sox7 reduced Wnt (see WNT1)/beta-catenin (see CTNNB1)- stimulated transcription. SOX7 is a potent activator of FGF3 transcription. It not only plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium but also blocks further differentiation into blood precursors. And it may be required for the survival of both hematopoietic and endothelial precursors during specification.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05013-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Colo320 whole cell, human PC-3 whole cell, human A431 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SOX7 using anti-SOX7 antibody (A05013-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Colo320 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX7 antigen affinity purified polyclonal antibody (Catalog # A05013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX7 at approximately 43 kDa. The expected band size for SOX7 is at 43 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SOX7 (Source: Uniprot.org, NCBI)
Gene Name
SOX7
Full Name
Transcription factor SOX-7
Weight
25847 MW
Alternative Names
Fms-related tyrosine kinase 3 ligand ;Flt3lg ; Sox7|SRY (sex determining region Y)-box 7|transcription factor SOX-7|SRY-box containing gene 7|mSOX7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX7, check out the SOX7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOX7 Antibody Picoband® (A05013-1)
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