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Product Info Summary
| SKU: | A07714-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SNURPORTIN1/SNUPN Antibody Picoband®
View all SNURPORTIN1 Antibodies
SKU/Catalog Number
A07714-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNURPORTIN1/SNUPN Antibody Picoband® catalog # A07714-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SNURPORTIN1/SNUPN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07714-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SNURPORTIN1/SNUPN recombinant protein (Position: M1-N360).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07714-1 is reactive to SNUPN in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
49229 MW
Background of SNURPORTIN1
Snurportin1 is a protein that in humans is encoded by the SNUPN gene. The nuclear import of the spliceosomal snRNPs U1, U2, U4 and U5, is dependent on the presence of a complex nuclear localization signal. The latter is composed of the 5'-2,2,7-terminal trimethylguanosine (m3G) cap structure of the U snRNA and the Sm core domain. The protein encoded by this gene interacts specifically with m3G-cap and functions as an snRNP-specific nuclear import receptor. Alternatively spliced transcript variants encoding the same protein have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07714-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human THP-1 whole cell, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue
ICC/IF: U87 cell
FCM: JK cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SNURPORTIN1/SNUPN using anti-SNURPORTIN1/SNUPN antibody (A07714-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNURPORTIN1/SNUPN antigen affinity purified polyclonal antibody (Catalog # A07714-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNURPORTIN1/SNUPN at approximately 41 kDa. The expected band size for SNURPORTIN1/SNUPN is at 41 kDa.
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Figure 2. IF analysis of SNURPORTIN1/SNUPN using anti-SNURPORTIN1/SNUPN antibody (A07714-1).
SNURPORTIN1/SNUPN was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNURPORTIN1/SNUPN Antibody (A07714-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of JK cells using anti-SNURPORTIN1/SNUPN antibody (A07714-1).
Overlay histogram showing JK cells stained with A07714-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNURPORTIN1/SNUPN Antibody (A07714-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SNUPN (Source: Uniprot.org, NCBI)
Gene Name
SNUPN
Full Name
Snurportin-1
Weight
49229 MW
Superfamily
snurportin family
Alternative Names
Protein Hook homolog 3; h-hook3; Hhk3; HOOK3 SNUPN KPNBL, RNUT1, Snurportin1 snurportin 1 snurportin-1|RNA U transporter 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNUPN, check out the SNUPN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNUPN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SNURPORTIN1/SNUPN Antibody Picoband® (A07714-1)
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