Product Info Summary
SKU: | A05519-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SMOX Antibody Picoband™
SKU/Catalog Number
A05519-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SMOX Antibody Picoband™ catalog # A05519-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SMOX Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05519-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SMOX recombinant protein (Position: E45-Q454).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05519-2 is reactive to SMOX in Human, Rat
Applications
A05519-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
69 kDa
Calculated molecular weight
48608 MW
Background of SMOX
Spermine oxidase is an enzyme that in humans is encoded by the SMOX gene. Polyamines are ubiquitous polycationic alkylamines which include spermine, spermidine, putrescine, and agmatine. These molecules participate in a broad range of cellular functions which include cell cycle modulation, scavenging reactive oxygen species, and the control of gene expression. These molecules also play important roles in neurotransmission through their regulation of cell-surface receptor activity, involvement in intracellular signalling pathways, and their putative roles as neurotransmitters. This gene encodes an FAD-containing enzyme that catalyzes the oxidation of spermine to spermadine and secondarily produces hydrogen peroxide. Multiple transcript variants encoding different isoenzymes have been identified for this gene, some of which have failed to demonstrate significant oxidase activity on natural polyamine substrates. The characterized isoenzymes have distinctive biochemical characteristics and substrate specificities, suggesting the existence of additional levels of complexity in polyamine catabolism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a05519 2 smox primary antibodies wb testing 1 a05519 2 smox primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of SMOX using anti-SMOX antibody (A05519-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMOX antigen affinity purified polyclonal antibody (Catalog # A05519-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMOX at approximately 69 kDa. The expected band size for SMOX is at 62 kDa.
![a05519 2 smox primary antibodies ihc testing 2 a05519 2 smox primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies ihc testing 3 a05519 2 smox primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human duodenal papilla adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies ihc testing 4 a05519 2 smox primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies ihc testing 5 a05519 2 smox primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies ihc testing 6 a05519 2 smox primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies ihc testing 7 a05519 2 smox primary antibodies ihc testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-7.jpg)
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Figure 7. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies ihc testing 8 a05519 2 smox primary antibodies ihc testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-8.jpg)
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Figure 8. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies ihc testing 9 a05519 2 smox primary antibodies ihc testing 9](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-9.jpg)
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Figure 9. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies ihc testing 10 a05519 2 smox primary antibodies ihc testing 10](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-10.jpg)
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Figure 10. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies ihc testing 11 a05519 2 smox primary antibodies ihc testing 11](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-ihc-testing-11.jpg)
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Figure 11. IHC analysis of SMOX using anti-SMOX antibody (A05519-2).
SMOX was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a05519 2 smox primary antibodies fcm testing 12 a05519 2 smox primary antibodies fcm testing 12](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-fcm-testing-12.png)
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Figure 12. Flow Cytometry analysis of HL-60 cells using anti-SMOX antibody (A05519-2).
Overlay histogram showing HL-60 cells stained with A05519-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOX Antibody (A05519-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a05519 2 smox primary antibodies fcm testing 13 a05519 2 smox primary antibodies fcm testing 13](https://www.bosterbio.com/media/catalog/product/a/0/a05519-2-smox-primary-antibodies-fcm-testing-13.png)
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Figure 13. Flow Cytometry analysis of MCF-7 cells using anti-SMOX antibody (A05519-2).
Overlay histogram showing MCF-7 cells stained with A05519-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOX Antibody (A05519-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SMOX (Source: Uniprot.org, NCBI)
Gene Name
SMOX
Full Name
Spermine oxidase
Weight
48608 MW
Superfamily
flavin monoamine oxidase family
Alternative Names
C20orf16; chromosome 20 open reading frame 16; EC 1.5.3.16; flavin containing amine oxidase; flavin-containing spermine oxidase; FLJ20746; MGC1010; PAO; PAO-1; PAOH1; Polyamine oxidase 1; putative cyclin G1 interacting protein; SMOdJ779E11.1; spermine oxidase SMOX C20orf16, PAO, PAO-1, PAO1, PAOH, PAOH1, SMO spermine oxidase spermine oxidase|flavin containing amine oxidase|flavin-containing spermine oxidase|polyamine oxidase 1|putative cyclin G1 interacting protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMOX, check out the SMOX Infographic
![SMOX infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMOX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMOX Antibody Picoband™ (A05519-2)
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