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Product Info Summary
| SKU: | A15467-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-SLC5A4 Antibody Picoband®
View all SGLT3/SLC5A4 Antibodies
SKU/Catalog Number
A15467-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC5A4 Antibody Picoband® catalog # A15467-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLC5A4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15467-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SLC5A4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A15467-2 is reactive to SLC5A4 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
24145 MW
Background of SGLT3/SLC5A4
The low affinity sodium-glucose cotransporter also known as the sodium/glucose cotransporter 3 (SGLT3) or solute carrier family 5 member 4 (SLC5A4) is a protein that in humans is encoded by the SLC5A4 gene. Predicted to enable glucose:sodium symporter activity and proton transmembrane transporter activity. Predicted to be involved in sodium ion transport. Predicted to act upstream of or within proton transmembrane transport. Predicted to be active in plasma membrane. Predicted to be integral component of membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15467-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human SH-SY5Y whole cell, human 293T whole cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC5A4 using anti-SLC5A4 antibody (A15467-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A4 antigen affinity purified polyclonal antibody (Catalog # A15467-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A4 at approximately 80 kDa. The expected band size for SLC5A4 is at 72 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-SLC5A4 antibody (A15467-2).
Overlay histogram showing 293T cells stained with A15467-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC5A4 Antibody (A15467-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SLC5A4 (Source: Uniprot.org, NCBI)
Gene Name
SLC5A4
Full Name
Solute carrier family 5 member 4
Weight
24145 MW
Superfamily
sodium:solute symporter (SSF) (TC 2.A.21) family
Alternative Names
Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489 SLC5A4 DJ90G24.4, SAAT1, SGLT3 solute carrier family 5 member 4 solute carrier family 5 member 4|Na(+)/glucose cotransporter 3|low affinity sodium-glucose cotransporter|sodium transporter|sodium/glucose cotransporter 3|solute carrier family 5 (glucose activated ion channel), member 4|solute carrier family 5 (low affinity glucose cotransporter), member 4|solute carrier family 5 (neutral amino acid transporters, system A), member 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC5A4, check out the SLC5A4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC5A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC5A4 Antibody Picoband® (A15467-2)
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