Product Info Summary
SKU: | PA2064 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband®
SKU/Catalog Number
PA2064
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody catalog # PA2064. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2064)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SLC2A5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2064 is reactive to SLC2A5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
92 kDa
Calculated molecular weight
54974 MW
Background of Glut5
SLC2A5, also known as GLUT5 (Glucose transporter 5), is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient. In humans the GLUT5 protein is encoded by the SLC2A5 gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2064 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: U87 Cell, 293T Cell, HT1080 Cell, SW620 Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-SLC2A5 antibody, PA2064, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: SW620 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For SLC2A5 (Source: Uniprot.org, NCBI)
Gene Name
SLC2A5
Full Name
Solute carrier family 2, facilitated glucose transporter member 5
Weight
54974 MW
Superfamily
major facilitator superfamily
Alternative Names
Solute carrier family 2, facilitated glucose transporter member 5;Fructose transporter;Glucose transporter type 5, small intestine;GLUT-5;SLC2A5;GLUT5; SLC2A5 GLUT-5, GLUT5 solute carrier family 2 member 5 solute carrier family 2, facilitated glucose transporter member 5|glucose transporter type 5, small intestine|glucose transporter-like protein 5|solute carrier family 2 (facilitated glucose/fructose transporter), member 5|testicular tissue protein Li 81
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC2A5, check out the SLC2A5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC2A5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband® (PA2064)
Hello CJ!
PA2064 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Sex differences in renal and metabolic responses to a high-fructose diet in mice
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Customer Q&As
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6 Customer Q&As for Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband®
Question
Do you have a BSA free version of anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064 available?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-16
Question
I was wanting to use your anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody for WB for human brain umbilical cord blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain umbilical cord blood identification?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
It shows on the product datasheet, PA2064 anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain umbilical cord blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-16
Question
Does anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064 work for WB with brain umbilical cord blood?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
According to the expression profile of brain umbilical cord blood, SLC2A5 is highly expressed in brain umbilical cord blood. So, it is likely that anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064 will work for WB with brain umbilical cord blood.
Boster Scientific Support
Answered: 2018-05-30
Question
Can you help my question with product PA2064, anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Parker
Verified customer
Asked: 2018-05-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2064 anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-21
Question
Would anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064 work on pig WB with mammary cancer?
Verified Customer
Verified customer
Asked: 2017-09-27
Answer
Our lab technicians have not tested anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig mammary cancer in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-27
Question
I would like to test anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064 on human brain umbilical cord blood for research purposes, then I may be interested in using anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Rodriguez
Verified customer
Asked: 2014-10-02
Answer
The products we sell, including anti-Glucose Transporter 5 GLUT5/SLC2A5 antibody PA2064, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-10-02