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Product Info Summary
| SKU: | M00433 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00433
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody catalog # M00433. Tested in WB application. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00433)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HCA-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SIRT2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00433 is reactive to SIRT2 in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
160-180 kDa
Calculated molecular weight
43182 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00433 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of SIRT2 expression in SH-SY5Y cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For SIRT2 (Source: Uniprot.org, NCBI)
Gene Name
SIRT2
Full Name
NAD-dependent protein deacetylase sirtuin-2
Weight
43182 MW
Superfamily
sirtuin family
Alternative Names
NAD-dependent protein deacetylase sirtuin-2;3.5.1.-;Regulatory protein SIR2 homolog 2;SIR2-like protein 2;SIRT2;SIR2L, SIR2L2; SIRT2 SIR2, SIR2L, SIR2L2 sirtuin 2 NAD-dependent protein deacetylase sirtuin-2|NAD-dependent deacetylase sirtuin-2|SIR2-like protein 2|regulatory protein SIR2 homolog 2|silent information regulator 2|sir2-related protein type 2|sirtuin type 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRT2, check out the SIRT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody (M00433)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433 available?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-31
Question
We have observed staining in human c1 segment of cervical spinal cord. Do you have any suggestions? Is anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody supposed to stain c1 segment of cervical spinal cord positively?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
According to literature c1 segment of cervical spinal cord does express SIRT2. According to Uniprot.org, SIRT2 is expressed in c1 segment of cervical spinal cord, testis, adrenal gland, brain lung, lung, brain, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SIRT2 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 10931946
Brain, Pubmed ID: 11226170
Brain, and Lung, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2019-10-30
Question
Is this M00433 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody reactive to the isotypes of SIRT2?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The immunogen of M00433 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody is A synthesized peptide derived from human SIRT2 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-23
Question
I have a question about product M00433, anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00433 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-12
Question
I was wanting to use your anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
It shows on the product datasheet, M00433 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-19
Question
My team were satisfied with the WB result of your anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody. However we have seen positive staining in c1 segment of cervical spinal cord perinuclear region. using this antibody. Is that expected? Could you tell me where is SIRT2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-01-14
Answer
From literature, c1 segment of cervical spinal cord does express SIRT2. Generally SIRT2 expresses in nucleus. cytoplasm, perinuclear region., isoform 1: cytoplasm, isoform 2: cytoplasm, isoform 5: cytoplasm. Regarding which tissues have SIRT2 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 10931946
Brain, Pubmed ID: 11226170
Brain, and Lung, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2019-01-14
Question
Is a blocking peptide available for product anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody (M00433)?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
We do provide the blocking peptide for product anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody (M00433). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-12
Question
I see that the anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-01
Question
Does M00433 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
It shows on the product datasheet, M00433 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-27
Question
My lab would like to test anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433 on rat testis for research purposes, then I may be interested in using anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
The products we sell, including anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-25
Question
Does anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433 work for WB with testis?
P. Jones
Verified customer
Asked: 2015-07-14
Answer
According to the expression profile of testis, SIRT2 is highly expressed in testis. So, it is likely that anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433 will work for WB with testis.
Boster Scientific Support
Answered: 2015-07-14
Question
Please see the WB image, lot number and protocol we used for testis using anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433. Please let me know if you require anything else.
H. Parker
Verified customer
Asked: 2015-06-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-01
Question
We are currently using anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on pig tissues as well?
J. Li
Verified customer
Asked: 2014-05-02
Answer
The anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody (M00433) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-02
